Anti-Keratin16 [LL025]
Invented by Prof Irene Leigh from Queen Mary University of London
Invented at Queen Mary University of London
- Datasheet
- References (13)
- Inventor Info
Info
| Catalogue Number | 151123 |
| Applications | IHC IF IP WB |
| Antigen/Gene or Protein Targets | Keratin 16 |
| Reactivity | Human |
| Relevance |
Monoclonal antibody used to detect keratin 16 mutations within epithelial tissues. Background and Research Application Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratin 16 is expressed in suprabasal keratinocytes of wounded epidermis. Keratin 16 suprabasal expression is found in situations of high cell turnover. Mutations in the gene encoding keratin16 are associated with the genetic skin disorders including pachyonychia congenita, non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus. LL025 reactivity was completely negative in normal breast tissue and non-invasive breast carcinoma. However, 10% of the invasive carcinomas tested were diffusely or focally positive with this antibody. |
| Host | Mouse |
| Immunogen | A synthetic peptide corresponding to an amino acid sequence of the carboxyl terminus of human keratin 16. |
| Immunogen UniProt ID | P08779 |
| Subclass | IgG1 |
| Myeloma Used | Sp2/0-Ag14 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest Anti- keratin 16 was completely negative in in-situ carcinomas. Immunoperoxidase is recommended for use on frozen sections. Concentration 1mg/ml as standard |
| Research Area | Cell Signaling & Signal Transduction |
References: 13 entries
Hughes BR et al. 1996. Br. J. Dermatol. 134(2):247-56.PMID: 8746337
Wong et al. 2019. Sci Rep. 9(1):18561. PMID: 31811191.
Frew et al. 2018. F1000Res. 7:1923. PMID: 31281635.
Boink et al. 2018. J Tissue Eng Regen Med. 12(2):e1088-e1097. PMID: 28388010.
Ojeh et al. 2017. PLoS One. 12(3):e0174389. PMID: 28350869.
Porter et al. 2000. Lab Invest. 80(11):1701-10. PMID: 11092530.
K15 expression implies lateral differentiation within stratified epithelial basal cells.
Europe PMC ID: 11092530
de Berker et al. 1995. Exp Dermatol. 4(6):350-6. PMID: 8608342.
Keratin expression in discoid lupus erythematosus.
Europe PMC ID: 8608342
Markey et al. 1992. Br J Dermatol. 126(2):154-60. PMID: 1371396.
Keratin expression in basal cell carcinomas.
Europe PMC ID: 1371396
Wetzels et al. 1991. Am J Pathol. 138(3):751-63. PMID: 1705754.
Basal cell-specific and hyperproliferation-related keratins in human breast cancer.
Europe PMC ID: 1705754
Add a reference
References: 13 entries
Hughes BR et al. 1996. Br. J. Dermatol. 134(2):247-56.PMID: 8746337
Wong et al. 2019. Sci Rep. 9(1):18561. PMID: 31811191.
Frew et al. 2018. F1000Res. 7:1923. PMID: 31281635.
Boink et al. 2018. J Tissue Eng Regen Med. 12(2):e1088-e1097. PMID: 28388010.
Ojeh et al. 2017. PLoS One. 12(3):e0174389. PMID: 28350869.
Porter et al. 2000. Lab Invest. 80(11):1701-10. PMID: 11092530.
K15 expression implies lateral differentiation within stratified epithelial basal cells.
de Berker et al. 1995. Exp Dermatol. 4(6):350-6. PMID: 8608342.
Keratin expression in discoid lupus erythematosus.
Markey et al. 1992. Br J Dermatol. 126(2):154-60. PMID: 1371396.
Keratin expression in basal cell carcinomas.
Wetzels et al. 1991. Am J Pathol. 138(3):751-63. PMID: 1705754.
Basal cell-specific and hyperproliferation-related keratins in human breast cancer.
Add a reference
