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Anti-MCSP [LHM 2]

Info

Catalogue Number 151118
Applications FACS IHC IF IP WB
Antigen/Gene or Protein Targets Melanoma associated Chondroitin Sulphate Proteoglycan (MCSP or NG2)
Reactivity Human
Relevance Monoclonal antibody against MCSP melanoma marker.

Background and Research Application
MCSP is a chondroitin surface proteoglycan, involved in stabilizing cell-substratum interactions during early events of cell spreading. MCSP is an integral membrane chondroitin sulphate proteoglycan expressed by human malignant melanoma cells.
Anti-MCSP (LHM2) antibody is a very good marker for melanoma (>90% stained). LHM2 also stains basal cell carcinomas and subpopulation of basal keratinocytes. LHM2 can be used as a diagnostic panel for immunopathology of malignant melanoma and potential candidates for immunoimaging and immunotherapy of disseminated melanoma. Potential applications of the LHM2 antibody include use for separation/isolation of epidermal stem cells, detection of stem cells in skin biopsies or epithelial cell cultures and optimisation of culture conditions to retain epidermal stem cell population in in vitro cultures.
Host Mouse
Immunogen A 375P cells crude extract.
Immunogen UniProt ID Q6UVK1
Subclass IgG1 kappa
Molecular Weight (kDa) 240
Myeloma Used NS0
Recommended Growing Conditions DMEM + 5% FCS
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
The originators of this antibody report that the MCSP is a cell surface marker specific for epidermal stem cells (J. Invest. Dermatol. in press).

Concentration
1mg/ml as standard
Research Area Adhesion, Cancer, Cell Type or Organelle Marker, Neurobiology

References

There are 12 reference entries for this reagent.

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References: 12 entries

Li et al. 2019. Theranostics. 9(17):5105-5121. PMID: 31410204.

Arrigoni et al. 2016. Adv Healthc Mater. 5(13):1617-26. PMID: 27191352.

Somaiah et al. 2012. Clin Cancer Res. 18(19):5479-88. PMID: 22855580.

The relationship between homologous recombination repair and the sensitivity of human epidermis to the size of daily doses over a 5-week course of breast radiotherapy.

Europe PMC ID: 22855580

Kinney et al. 2012. Ann Neurol. 71(3):397-406. PMID: 22451205.

Pierce et al. 2011. Aging Cell. 10(6):1032-7. PMID: 21943306.

Ghali et al. 2004. J Invest Dermatol. 122(2):433-42. PMID: 15009727.

Epidermal and hair follicle progenitor cells express melanoma-associated chondroitin sulfate proteoglycan core protein.

Europe PMC ID: 15009727

Kupsch et al. 1999. Clin Cancer Res. 5(4):925-31. PMID: 10213230.

Isolation of human tumor-specific antibodies by selection of an antibody phage library on melanoma cells.

Europe PMC ID: 10213230

Kupsch et al. 1995. Melanoma Res. 5(6):403-11. PMID: 8589614.

Generation and selection of monoclonal antibodies, single-chain Fv and antibody fusion phage specific for human melanoma-associated antigens.

Europe PMC ID: 8589614


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References: 12 entries

Li et al. 2019. Theranostics. 9(17):5105-5121. PMID: 31410204.

Arrigoni et al. 2016. Adv Healthc Mater. 5(13):1617-26. PMID: 27191352.

Somaiah et al. 2012. Clin Cancer Res. 18(19):5479-88. PMID: 22855580.

The relationship between homologous recombination repair and the sensitivity of human epidermis to the size of daily doses over a 5-week course of breast radiotherapy.

Kinney et al. 2012. Ann Neurol. 71(3):397-406. PMID: 22451205.

Pierce et al. 2011. Aging Cell. 10(6):1032-7. PMID: 21943306.

Ghali et al. 2004. J Invest Dermatol. 122(2):433-42. PMID: 15009727.

Epidermal and hair follicle progenitor cells express melanoma-associated chondroitin sulfate proteoglycan core protein.

Kupsch et al. 1999. Clin Cancer Res. 5(4):925-31. PMID: 10213230.

Isolation of human tumor-specific antibodies by selection of an antibody phage library on melanoma cells.

Kupsch et al. 1995. Melanoma Res. 5(6):403-11. PMID: 8589614.

Generation and selection of monoclonal antibodies, single-chain Fv and antibody fusion phage specific for human melanoma-associated antigens.


Add a reference

Inventor Information

No inventors are currently linked to this reagent.

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