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Anti-Keratin5 & 14 [LH8]


Catalogue Number 151116
Applications IHC IF
Antigen/Gene or Protein Targets Keratin 5 & 14
Reactivity Human
Relevance Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratins 5 and 14 are the predominant keratins in basal layer keratinocytes in stratified epithelia. Their expression is restricted to basal cells and are found on all keratinocytes. Keratin 14 is one of two keratins that distinguish stratifying epithelial cell types from simple epithelial cell types. Mutations in keratin 5 or 14 cause the skin blistering disorder epidermolysis bullosa simplex. LH8 may be useful as a basal cell marker and myoepithelial marker in stratified squamous or mixed epithelia.
Host Mouse
Immunogen The 50,000 Mr band (including K14) was cut from an SDS-polyacrylamide gel and Triton X-100 extracted cytoskeleton pellet from cultured human foreskin keratinocytes.
Subclass IgM
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Notes For immunohistochemistry, use unfixed frozen sections with this antibody.
Research Area Cell Signaling & Signal Transduction

References: 2 entries

Prince et al. 2007. Proc Natl Acad Sci U S A. 104(3):973-8. PMID: 17210912.

de Berker et al. 1995. Exp Dermatol. 4(6):350-6. PMID: 8608342.

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