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Anti-Keratin10 [LH2]


Catalogue Number 151115
Applications FACS IHC IF IP WB
Antigen/Gene or Protein Targets Keratin 10
Synonyms BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10
Human Entrez Gene ID 3858
Human SwissProt P13645
Human Unigene 99936
Human Gene Symbol KRT10
Human Chromosome Location 17q21.2
Reactivity Human and Mouse
Relevance LH2 recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10). Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratin 10 is differentiation specific keratin that is one of the predominant keratins in suprabasal keratinocytes in stratified epthelia. Keratin 10 is a suprabasal marker of differentiation in stratified squamous epithelia and is present on all suprabasal keratinocytes. Mutations in keratin 10 cause epidermolytic hyperkeratosis. LH2 does not react with any tumour tissue regardless of histological classification.
Host Mouse
Immunogen extract of skin from psoriasis patient
Subclass IgG1 kappa
Positive Control Extract of skin from psoriasis patient
Molecular Weight (kDa) 56.5kDa
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Notes LH2 is monospecific and has high affinity.
Cellular Localization: Cytoplasmic
Suggested Dilutions: Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); Immunohistology (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Research Area Cell Signaling & Signal Transduction

References: 5 entries

Barrott et al. 2011. Proc Natl Acad Sci U S A. 108(31):12752-7. PMID: 21768372.

Porter et al. 2000. Lab Invest. 80(11):1701-10. PMID: 11092530.

Reichelt et al. 1997. J Cell Sci. 110 ( Pt 18):2175-86. PMID: 9378767.

Markey et al. 1991. J Invest Dermatol. 97(5):763-70. PMID: 1717607.

Broekaert et al. 1990. Arch Dermatol Res. 282(6):383-91. PMID: 1701984.

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