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Catalogue Number 151106
Applications ELISA IHC RIA
Antigen/Gene or Protein Targets Placental alkaline phosphatase (PLAP)
Reactivity Human
Relevance Monoclonal antibody with potential use in detection of ovarian and testicular tumours via detection of PLAP.

Background and Research Application
Human placental alkaline phosphatase (PLAP) is a membrane bound glycosylated phosphodiesterase normally synthesised by syncytiotrophoblast from the 12th week of pregnancy. Since its identification as an oncofoetal antigen, it has been found to be expressed by malignant tumours of germ cell and non-germ cell origin. The detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer. HD 11F7 has potential uses in the diagnosis of ovarian and testicular tumours. This antibody detects both Regan and Nagao isoenzymes.
The placental-specific isozyme of Alkaline Phosphatase (PLAP), also referred to as the heat-stable form, is found in trophoblast cells of normal human mature placenta, seminomas of testis and ovarian carcinomas. It is closely related to the intestinal form of the enzyme as well as to the placental-like form.
Anti-PLAP was created for use in immunoscintigraphy or therapy.
Host Mouse
Immunogen Fresh Hep-2 cells followed by booster injections of fresh Hep-2 cells that had their surface expression of PLAP boosted in culture.
Immunogen UniProt ID P05187
Subclass IgG2b
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Notes
RECENTLY UPDATED
Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
This antibody also showed faster binding in vivo, in radioimmunolocalisation studies in Hep-2 xenograft bearing nude mice, compared to other antibodies produced in the same experiment.
PLAP is reliable only in non-smokers, as smoking interferes with measurement of PLAP, since serum concentrations of PLAP are increased up to 10-fold in smokers.

Concentration
1mg/ml as standard
Research Area Cancer, Cell Type or Organelle Marker

References

There are 3 reference entries for this reagent.

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References: 3 entries

Fitzgerald et al. 2018. Am J Reprod Immunol. 80(1):e12860. PMID: 29726582.

Durbin et al. 1988. Int J Cancer Suppl. 2:59-66. PMID: 3162446.

Monoclonal antibodies to placental alkaline phosphatase: preclinical evaluation in a human xenograft tumour model of F(ab')2 and Fab fragments.

Europe PMC ID: 3162446


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References: 3 entries

Fitzgerald et al. 2018. Am J Reprod Immunol. 80(1):e12860. PMID: 29726582.

Durbin et al. 1988. Int J Cancer Suppl. 2:59-66. PMID: 3162446.

Monoclonal antibodies to placental alkaline phosphatase: preclinical evaluation in a human xenograft tumour model of F(ab')2 and Fab fragments.


Add a reference