Anti-Fibronectin [Fn-3]
Invented by Prof Joyce Taylor-Papadimitriou from King's College London
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (6)
- Inventor Info
Info
| Catalogue Number | 151098 |
| Applications | IHC IF IP WB |
| Antigen/Gene or Protein Targets | Fibronectin |
| Reactivity | Human |
| Relevance |
Monoclonal antibody capable of differentiating between cellular and plasma fibronectin, in healthy cells or tumours. Background and Research Application Fibronectin is a widely expressed extracellular matrix glycoprotein that is exists in two forms, a soluble plasma form and a less soluble cellular form. Fibronectin is involved in cell adhesion, maintenance of cell shape and migration processes including embryogenesis, wound healing, blood coagulation, host defence, and metastasis. Cellular fibronectin is often found in the stroma of malignant tumours. Fn-3 can distinguish between human cellular and plasma fibronectin, thus making it possible to analyse the origin and levels of different types of fibronectin in normal human tissues and their related tumours. |
| Host | Mouse |
| Immunogen | FR5 cells, derived by SV40 transformation of human mammary epithelial cells. |
| Immunogen UniProt ID | P02751 |
| Subclass | IgG1 |
| Myeloma Used | P3X63Ag8.653 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Strain | Balb/c |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest The antigenic site recognized by FN-3 is lost after limited proteolysis of cellular fibronectin with alpha-chymotrypsin but not trypsin. Concentration 1mg/ml as standard |
| Research Area | Adhesion, Cancer, Cardiovascular, Cell Signaling & Signal Transduction, Developmental Biology, Stem Cell Biology |
References: 6 entries
Bambang et al. 2013. Oncogene. 32(10):1240-51. PMID: 22543584.
Endoplasmic reticulum protein 29 regulates epithelial cell integrity during the mesenchymal-epithelial transition in breast cancer cells.
Europe PMC ID: 22543584
Cseh et al. 2010. J Cell Sci. 123(Pt 22):3989-99. PMID: 20980391.
Autocrine fibronectin directs matrix assembly and crosstalk between cell-matrix and cell-cell adhesion in vascular endothelial cells.
Europe PMC ID: 20980391
Keen et al. 1984. Mol Biol Med. 2(1):15-27. PMID: 6083431.
Monoclonal antibodies that distinguish between human cellular and plasma fibronectin.
Europe PMC ID: 6083431
Add a reference
References: 6 entries
Bambang et al. 2013. Oncogene. 32(10):1240-51. PMID: 22543584.
Endoplasmic reticulum protein 29 regulates epithelial cell integrity during the mesenchymal-epithelial transition in breast cancer cells.
Cseh et al. 2010. J Cell Sci. 123(Pt 22):3989-99. PMID: 20980391.
Autocrine fibronectin directs matrix assembly and crosstalk between cell-matrix and cell-cell adhesion in vascular endothelial cells.
Keen et al. 1984. Mol Biol Med. 2(1):15-27. PMID: 6083431.
Monoclonal antibodies that distinguish between human cellular and plasma fibronectin.
Add a reference
