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Monoclonal Antibodies

Anti-Beta Galactosidase [DC1 4C7]

Invented by Prof Walter Bodmer from University of Oxford
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
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Adapted from Smith et al. 2012. PLoS One. 7(12):e52335. PMID: 23272238. Figure. Heterogeneous cellular phenotypes and reduced proliferation rates of RCCS aggregates. A–C –Immunohistochemistry against Ki67 for U87, PFSK-1 and primary HGG respectively demonstrates proliferative cells in the rim and/or rim/core interface of aggregates at similar frequencies to primary tumor. D–F – Immunohistochemistry against P16 in a KNS42 aggregate core (D), KNS42 rim (E) and in a primary HGG (F) showing a higher proportion of cells staining positive in the aggregate core compared to the rim and which is comparable to the primary tumor. G–I – Immunohistochemistry against beta-galactosidase in a KNS42 aggregate core (G), KNS42 aggregate rim (H) and in a primary HGG (I) showing that significant staining for senescence markers occurs in both resected tumors and aggregate cores. J–L – Immunofluorescence against beta-galactosidase in KNS42 aggregates, KNS42 2D monolayer cells and U87 2D monolayer cells. M&N – Alamar Blue assay proliferation rates in U87 (J) and KNS42 (K) comparing 2D and RCCS culture demonstrating significantly decreased proliferation in 3D compared to 2D for both cell lines. Scale bars in A–I and K–L are 25 µm. Scale bar in J is 100 µm.
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Catalogue Number 151086
Applications ELISA IHC IF WB
Antigen/Gene or Protein Targets Beta-galactosidase
Reactivity E.coli
Relevance This antibody is useful for detection of beta-galactosidase fusion proteins.
Host Mouse
Immunogen ß-galactosidase EC.3.2.1.23 from E.coli
Subclass IgG1
Molecular Weight (kDa) 130
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Research Area Bacteriology, Cell Type or Organelle Marker

References

There are 8 reference entries for this reagent.

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References: 8 entries

Recapitulation of tumor heterogeneity and molecular signatures in a 3D brain cancer model with decreased sensitivity to histone deacetylase inhibition.

Europe PMC ID: 23272238

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Smith et al. 2012. PLoS One. 7(12):e52335. PMID: 23272238.

View  

Büchmann-Møller et al. 2009. Dev Biol. 330(2):329-38. PMID: 19361496.

View  

Multiple lineage-specific roles of Smad4 during neural crest development.

Europe PMC ID: 19361496

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Li et al. 2005. Genesis. 41(2):73-80. PMID: 15682388.

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Rhodopsin-iCre transgenic mouse line for Cre-mediated rod-specific gene targeting.

Europe PMC ID: 15682388

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Durbin et al. 1987. J Immunol Methods. 97(1):19-27. PMID: 3102611.

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A sensitive micro-immunoassay using beta-galactosidase/anti-beta-galactosidase complexes.

Europe PMC ID: 3102611

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References: 8 entries

Recapitulation of tumor heterogeneity and molecular signatures in a 3D brain cancer model with decreased sensitivity to histone deacetylase inhibition.

View  

Smith et al. 2012. PLoS One. 7(12):e52335. PMID: 23272238.

View  

Büchmann-Møller et al. 2009. Dev Biol. 330(2):329-38. PMID: 19361496.

View  

Multiple lineage-specific roles of Smad4 during neural crest development.

View  

Li et al. 2005. Genesis. 41(2):73-80. PMID: 15682388.

View  

Rhodopsin-iCre transgenic mouse line for Cre-mediated rod-specific gene targeting.

View  

Durbin et al. 1987. J Immunol Methods. 97(1):19-27. PMID: 3102611.

View  

A sensitive micro-immunoassay using beta-galactosidase/anti-beta-galactosidase complexes.

View  

Add a reference

Inventor Information