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Anti-CAT [CAT-1]


Catalogue Number 151085
Applications IHC IF WB
Antigen/Gene or Protein Targets Chloramphenicol acetyl transferase (CAT)
Reactivity N/A
Relevance Bacterial chloramphenicol acetyl transferase (CAT) is an enzyme that catalyzes the inactivation of the antibiotic, chloramphenicol, by acetylation and subsequently confers bacterial resistance to the antibiotic. CAT, being a stable prokaryotic enzyme, is often used as a reporter gene in transfection assays developed for eukaryotic promoters. Quantification of reporter gene expressions, such as that of CAT, can be correlated to the transcriptional functions of the target sequence. Thus, antibodies directed against CAT can be used for the study of gene sequences that are fused to the CAT reporter gene.
Host Mouse
Immunogen Full length Chloramphenicol acetyl transferase
Subclass IgG1
Molecular Weight (kDa) 26000
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 5% FCS
Research Area Bacteriology, Cell Type or Organelle Marker, Gene Expression

References: 1 entry

Kuang et al. 2007. Am J Physiol Lung Cell Mol Physiol. 292(4):L944-52. PMID: 17209135.

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