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Catalogue Number 151085
Applications
RECENTLY UPDATED
IHC IF WB
Antigen/Gene or Protein Targets Chloramphenicol acetyl transferase (CAT)
Reactivity N/A
Relevance Bacterial chloramphenicol acetyl transferase (CAT) is an enzyme that catalyzes the inactivation of the antibiotic, chloramphenicol, by acetylation and subsequently confers bacterial resistance to the antibiotic. CAT, being a stable prokaryotic enzyme, is often used as a reporter gene in transfection assays developed for eukaryotic promoters. Quantification of reporter gene expressions, such as that of CAT, can be correlated to the transcriptional functions of the target sequence. Thus, antibodies directed against CAT can be used for the study of gene sequences that are fused to the CAT reporter gene.
Host Mouse
Immunogen Full length Chloramphenicol acetyl transferase
Subclass IgG1
Molecular Weight (kDa) 26000
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 5% FCS
Research Area Bacteriology, Cell Type or Organelle Marker, Gene Expression

References

There are 11 reference entries for this reagent.

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References: 11 entries
RECENTLY UPDATED

Augusto et al. 2019. PLoS Pathog. 15(6):e1007746. PMID: 31194856.

Persson et al. 2017. Am J Physiol Renal Physiol. 312(2):F278-F283. PMID: 27927650.

Mitchell et al. 2017. J Int Soc Sports Nutr. 14:17. PMID: 28603468.

WB

Sheen et al. 2016. Front Cell Neurosci. 10:167. PMID: 27445694.

WB

Kishikawa et al. 2015. Oncotarget. 6(10):8339-52. PMID: 25826076.

Wang et al. 2014. Biol Reprod. 91(3):59. PMID: 25061098.

Wang et al. 2014. Biol Reprod. 90(4):84. PMID: 24648395.

Battisti et al. 2012. Am J Respir Cell Mol Biol. 46(4):498-506. PMID: 22074703.

Barilli et al. 2011. J Leukoc Biol. 90(2):293-303. PMID: 21586674.

WB

Kuang et al. 2007. Am J Physiol Lung Cell Mol Physiol. 292(4):L944-52. PMID: 17209135.

Activation of elastin transcription by transforming growth factor-beta in human lung fibroblasts.

Europe PMC ID: 17209135


Add a reference

References: 11 entries
RECENTLY UPDATED

Augusto et al. 2019. PLoS Pathog. 15(6):e1007746. PMID: 31194856.

Persson et al. 2017. Am J Physiol Renal Physiol. 312(2):F278-F283. PMID: 27927650.

Mitchell et al. 2017. J Int Soc Sports Nutr. 14:17. PMID: 28603468.

WB

Sheen et al. 2016. Front Cell Neurosci. 10:167. PMID: 27445694.

WB

Kishikawa et al. 2015. Oncotarget. 6(10):8339-52. PMID: 25826076.

Wang et al. 2014. Biol Reprod. 91(3):59. PMID: 25061098.

Wang et al. 2014. Biol Reprod. 90(4):84. PMID: 24648395.

Battisti et al. 2012. Am J Respir Cell Mol Biol. 46(4):498-506. PMID: 22074703.

Barilli et al. 2011. J Leukoc Biol. 90(2):293-303. PMID: 21586674.

WB

Kuang et al. 2007. Am J Physiol Lung Cell Mol Physiol. 292(4):L944-52. PMID: 17209135.

Activation of elastin transcription by transforming growth factor-beta in human lung fibroblasts.


Add a reference