Anti-CAT [CAT-1]
Invented by Julian Gannon from The Francis Crick Institute
Invented at Cancer Research UK London Research Institute: Clare Hall Laboratories
- Datasheet
- References (11)
- Inventor Info
Info
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| Catalogue Number | 151085 |
| Applications | IHC IF WB |
| Antigen/Gene or Protein Targets | Chloramphenicol acetyl transferase (CAT) |
| Reactivity | N/A |
| Relevance | Bacterial chloramphenicol acetyl transferase (CAT) is an enzyme that catalyzes the inactivation of the antibiotic, chloramphenicol, by acetylation and subsequently confers bacterial resistance to the antibiotic. CAT, being a stable prokaryotic enzyme, is often used as a reporter gene in transfection assays developed for eukaryotic promoters. Quantification of reporter gene expressions, such as that of CAT, can be correlated to the transcriptional functions of the target sequence. Thus, antibodies directed against CAT can be used for the study of gene sequences that are fused to the CAT reporter gene. |
| Host | Mouse |
| Immunogen | Full length Chloramphenicol acetyl transferase |
| Subclass | IgG1 |
| Molecular Weight (kDa) | 26000 |
| Myeloma Used | Sp2/0-Ag14 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Research Area | Bacteriology, Cell Type or Organelle Marker, Gene Expression |
References: 11 entries
Augusto et al. 2019. PLoS Pathog. 15(6):e1007746. PMID: 31194856.
Persson et al. 2017. Am J Physiol Renal Physiol. 312(2):F278-F283. PMID: 27927650.
Mitchell et al. 2017. J Int Soc Sports Nutr. 14:17. PMID: 28603468.
WB
Sheen et al. 2016. Front Cell Neurosci. 10:167. PMID: 27445694.
WB
Kishikawa et al. 2015. Oncotarget. 6(10):8339-52. PMID: 25826076.
Wang et al. 2014. Biol Reprod. 91(3):59. PMID: 25061098.
Wang et al. 2014. Biol Reprod. 90(4):84. PMID: 24648395.
Battisti et al. 2012. Am J Respir Cell Mol Biol. 46(4):498-506. PMID: 22074703.
Barilli et al. 2011. J Leukoc Biol. 90(2):293-303. PMID: 21586674.
WB
Kuang et al. 2007. Am J Physiol Lung Cell Mol Physiol. 292(4):L944-52. PMID: 17209135.
Activation of elastin transcription by transforming growth factor-beta in human lung fibroblasts.
Europe PMC ID: 17209135
Add a reference
References: 11 entries
Augusto et al. 2019. PLoS Pathog. 15(6):e1007746. PMID: 31194856.
Persson et al. 2017. Am J Physiol Renal Physiol. 312(2):F278-F283. PMID: 27927650.
Mitchell et al. 2017. J Int Soc Sports Nutr. 14:17. PMID: 28603468.
WB
Sheen et al. 2016. Front Cell Neurosci. 10:167. PMID: 27445694.
WB
Kishikawa et al. 2015. Oncotarget. 6(10):8339-52. PMID: 25826076.
Wang et al. 2014. Biol Reprod. 91(3):59. PMID: 25061098.
Wang et al. 2014. Biol Reprod. 90(4):84. PMID: 24648395.
Battisti et al. 2012. Am J Respir Cell Mol Biol. 46(4):498-506. PMID: 22074703.
Barilli et al. 2011. J Leukoc Biol. 90(2):293-303. PMID: 21586674.
WB
Kuang et al. 2007. Am J Physiol Lung Cell Mol Physiol. 292(4):L944-52. PMID: 17209135.
Activation of elastin transcription by transforming growth factor-beta in human lung fibroblasts.
Add a reference
