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Anti-BCRABL [7c6]

Info

Catalogue Number 151062
Applications IF IP WB
Antigen/Gene or Protein Targets BCRABL
Reactivity Human
Relevance Monoclonal antibody directed against BCR-ABL oncoprotein.

Background and Research Application
The BCR gene is involved in the 9:22 translocation that generates the Philadelphia chromosome both in chronic myeloid leukaemia (CML) and in a proportion of cases of acute lymphocytic leukaemia (ALL). A 5' bcr sequence becomes fused to an abl sequence (including tyrosine kinase domain sequences) from chromosome 9. This results in the production of a chimeric BCR-ABL oncoprotein with constitutively active tyrosine kinase activity.
Anti-BCRABL antibody may be useful for studies of the oncogene and have potential diagnostic and prognostic applications, particularly within leukaemia. This antibody is specific for the SSINEEITPRRQS epitope of BCR (residues 686-696), and as such is able to recognise full-length BCR and the p210 form of the BCR-ABL fusion protein.
Host Mouse
Immunogen Bcr686 thyroglobulin conjugate
Immunogen UniProt ID Q62199
Subclass IgG2a
Molecular Weight (kDa) 210
Myeloma Used P3X63Ag8
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
This antibody can detect BCR-ABL protein and full length BCR.

Concentration
1mg/ml as standard
Research Area Cancer, Cell Signaling & Signal Transduction, Epigenetics & Nuclear Signalling

References

There are 7 reference entries for this reagent.

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References: 7 entries

Mallery et al. 2017. Cancer Prev Res (Phila). 10(1):76-88. PMID: 27756753.

Dhut et al. 1990. Leukemia. 4(11):745-50. PMID: 2232885.

BCR-ABL and BCR proteins: biochemical characterization and localization.

Europe PMC ID: 2232885

Dhut et al. 1988. Oncogene. 3(5):561-6. PMID: 3078961.

Identification of two normal bcr gene products in the cytoplasm.

Europe PMC ID: 3078961

Walker et al. 1987. Nature. 329(6142):851-3. PMID: 3478595.

Novel chimaeric protein expressed in Philadelphia positive acute lymphoblastic leukaemia.

Europe PMC ID: 3478595


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References: 7 entries

Mallery et al. 2017. Cancer Prev Res (Phila). 10(1):76-88. PMID: 27756753.

Dhut et al. 1990. Leukemia. 4(11):745-50. PMID: 2232885.

BCR-ABL and BCR proteins: biochemical characterization and localization.

Dhut et al. 1988. Oncogene. 3(5):561-6. PMID: 3078961.

Identification of two normal bcr gene products in the cytoplasm.

Walker et al. 1987. Nature. 329(6142):851-3. PMID: 3478595.

Novel chimaeric protein expressed in Philadelphia positive acute lymphoblastic leukaemia.


Add a reference

Inventor Information

No inventors are currently linked to this reagent.

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