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Monoclonal Antibodies

Anti-Cdc13 [6F11/2]

Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
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Adapted from Koch et al. 2012. ACS Chem Biol; 7(4):723-31. PMID: 22264160. Figure. Immunoblot of whole cell extracts at the time point of release into mitosis (5 h) and 17 min after the release (5 h 17 min), when cells had reached mitosis. Shown are the Ark1-specific phosphorylations on Ark1-Thr244 (Ark1-ph21,22), condensin subunit Cnd2-S5 (Cnd2-ph23,24), and Mra1-S9 (Mra1-ph23). Presence of the MPM2 phosphoepitope26 and cyclin B (Cdc13) indicates that the inhibitor-treated cells were in mitosis. Tubulin serves as loading control.
Adapted from Decottignies et al. 2001. J Cell Sci; 114(Pt 14):2627-40. PMID: 11683390. Figure. Analysis of cdc2-YFP protein. Cdc13p (tagged or untagged) was detected in total cell extracts (lanes 1,2) or immunoprecipitated complexes (lanes 3,4) by western blot using anti-cdc13p mAb. Lanes 1,2, 50 μg of cell extract proteins. Lanes 3,4, immunoprecipitation.
Adapted from Rothe et al. 2017. J Cell Sci; 130(23):4028-4037. PMID: 29046339. Figure. Cdc13 protein level is specifically downregulated in response to UV. Wild-type (wt), rad3Δ and cdc25-6PK strains were synchronized in G2 phase before irradiation with UV. Samples were collected at the indicated time points. Immunoblots of wt (A) and rad3Δ (B) strains showing Cdc13 protein level in control and UV-treated samples
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Catalogue Number 151061
Applications IHC WB
Antigen/Gene or Protein Targets Cell-division cycle protein 13 (cdc13)
Synonyms Cell division control protein 13; YDL220C
Reactivity Schizosaccharomyces pombe
Relevance Cdc13 is a fission yeast (S. pombe) B-type M-phase cyclin. Cdc13 binds to Cdk1 (cdc2), and the resulting Cdk1-Cdc13 complex controls the G2/M transition of the cell cycle. 6F11/2 can be used for detecting Cdc13 and associated Cdk1 kinase activation.
Host Mouse
Immunogen Full length cdc13
Subclass IgG2a
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Research Area Epigenetics & Nuclear Signalling

References

There are 7 reference entries for this reagent.

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References: 7 entries

Rothe et al. 2017. J Cell Sci. 130(23):4028-4037. PMID: 29046339.

WB

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Koch et al. 2012. ACS Chem Biol. 7(4):723-31. PMID: 22264160.

WB

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A chemical genetic approach for covalent inhibition of analogue-sensitive aurora kinase.

Europe PMC ID: 22264160

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Okuzaki et al. 2010. Cell Cycle. 9(18):3751-60. PMID: 20855961.

WB

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Spo5 phosphorylation is essential for its own timely degradation and for successful meiosis in Schizosaccharomyces pombe.

Europe PMC ID: 20855961

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Decottignies et al. 2001. J Cell Sci. 114(Pt 14):2627-40. PMID: 11683390.

WB

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In vivo localisation of fission yeast cyclin-dependent kinase cdc2p and cyclin B cdc13p during mitosis and meiosis.

Europe PMC ID: 11683390

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References: 7 entries

Rothe et al. 2017. J Cell Sci. 130(23):4028-4037. PMID: 29046339.

WB

View  

Koch et al. 2012. ACS Chem Biol. 7(4):723-31. PMID: 22264160.

WB

View  

A chemical genetic approach for covalent inhibition of analogue-sensitive aurora kinase.

View  

Okuzaki et al. 2010. Cell Cycle. 9(18):3751-60. PMID: 20855961.

WB

View  

Spo5 phosphorylation is essential for its own timely degradation and for successful meiosis in Schizosaccharomyces pombe.

View  

Decottignies et al. 2001. J Cell Sci. 114(Pt 14):2627-40. PMID: 11683390.

WB

View  

In vivo localisation of fission yeast cyclin-dependent kinase cdc2p and cyclin B cdc13p during mitosis and meiosis.

View  

Add a reference

Inventor Information