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|Applications||ELISA FACS IHC IP WB|
|Antigen/Gene or Protein Targets||S100A8 (MRP-8) & S100A9 (MRP-14)|
|Relevance||S100A8 (MRP-8) and S100A9 (MRP-14) are cytosolic calcium-binding proteins of 8kDa and 14kDa that form a heterodimer. S100A8 and S100A9 are expressed in secretory and inflamed keratinocytes, peripheral blood monocytes, neutrophils and has been described in platelets, dendritic cells and some T cell types. Expression is lost on tissue maturation of monocytes to macrophages. S100A9 may be associated with monocyte and neutrophil activation and the accumulation of these cells in inflammatory sites.|
|Immunogen||Acute monocytic leukaemia cells.|
|Positive Control||This antibody was used to detect S100A8/S100A9 in neutrophil extracts (Hogg et al., 1989) This antibody was used to detect S100A8/S100A9 in Human monocyte and neutrophil extracts (Edgeworth et al., 1991)|
|Recommended Growing Conditions||DMEM + 5% FCS|
|Research Area||Cell Cycle, Epigenetics & Nuclear Signalling|
References: 4 entries
Ryckman et al. 2003. Arthritis Rheum. 48(8):2310-20. PMID: 12905486.
Hessian et al. 2001. Eur J Biochem. 268(2):353-63. PMID: 11168370.
Edgeworth et al. 1991. J Biol Chem. 266(12):7706-13. PMID: 2019594.
Hogg et al. 1989. Eur J Immunol. 19(6):1053-61. PMID: 2666142.
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