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Monoclonal Antibodies

Anti-LewisX [28]

Invented by Prof Nancy Hogg from Cancer Research UK London Research Institute: Lincoln's Inn Fields
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
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Adapted from Hogg et al. 1984. Immunology. 53(4):753-67. PMID: 6389324. Figure. (e) One 28-positive neutrophil in the monocyte preparation; (f) monocytes exposed to 28 shown by phase microscopy with one 28-positive neutrophil (large arrow). Magnification x 1275.
Adapted from Hogg et al. 1984. Immunology; 53(4):753-67. PMID: 6389324. Figure. Indirect immunoperoxidase staining with mAb 28 (b) tonsil: 28-positive cells in T-cell areas and concentrated at interface with capsule (d) kidney: 28-positive staining of distal convoluted tubules. Sections counterstained with haematoxylin.
Adapted from Hogg et al. 1984. Immunology. 53(4):753-67. PMID: 6389324. Figure. Monoclonal antibody immunoprecipitates from 125I-labelled monocyte and granulocyte lysates identified by 7.5% SDS-polyacrylamide gel electrophoresis after sample reduction. Positive reactions are indicated by arrows. Control preparations were incubated with mAb 5-5. Molecular weight markers were myosin, 200,000; fi-galactosidase, 130,000; phosphorylase b, 95,000; transferrin, 78,000; bovine albumin, 68,000; ovalbumin 45,000; glyceraldehyde 3-phosphate dehydrogenase 34,000; light chain, 25,000 cytochrome c, 12,500.
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Catalogue Number 151041
Applications FACS IHC IP
Antigen/Gene or Protein Targets Lewis X (CD15)
Synonyms Fucosyltransferase 4; Galactoside 3-L-Fucosyltransferase; ELAM-1 Ligand Fucosyltransferase; FUC-TIV; FCT3A; ELFT; Stage-Specific Embryonic Antigen; Alpha (1,3) Fucosyltransferase; EC 2.4.1.65; Lewis X; SSEA-1; FUTIV; CD15; LeX
Reactivity Human
Relevance Lewis X (CD15) is a branched pentasaccharide found on neutrophils, eosinophils and monocytes. Lewis X is distributed abnormally in myeloid leukeamias and is commonly used in the diagnosis of Hodgkin’s disease. It can also be used for analysis of myeloid leukaemias and studies of myeloid differentiation.
Host Mouse
Immunogen Monocytes, separated from other peripheral blood leucocytes (PBL) on fibronectin plates.
Subclass IgM
Molecular Weight (kDa) 83
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Notes Clone 28 does not activate complement.
Research Area Cancer, Immunology

References

There are 4 reference entries for this reagent.

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References: 4 entries

Hogg et al. 1984. Immunology. 53(4):753-67. PMID: 6389324.

IHC IP

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Monoclonal antibodies specific for human monocytes, granulocytes and endothelium.

Europe PMC ID: 6389324

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Linch et al. 1984. Blood. 63(3):566-73. PMID: 6365201.

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Monoclonal antibodies differentiating between monocytic and nonmonocytic variants of AML.

Europe PMC ID: 6365201

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Add a reference

References: 4 entries

Hogg et al. 1984. Immunology. 53(4):753-67. PMID: 6389324.

IHC IP

View  

Monoclonal antibodies specific for human monocytes, granulocytes and endothelium.

View  

Linch et al. 1984. Blood. 63(3):566-73. PMID: 6365201.

View  

Monoclonal antibodies differentiating between monocytic and nonmonocytic variants of AML.

View  

Add a reference

Inventor Information