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Anti-IL12 [1-1A4]

Info

Catalogue Number 151022
Applications ELISA IHC RIA WB
Antigen/Gene or Protein Targets Interleukin-12 (IL12) p40 subunit
Synonyms Interleukin 12; Cytotoxic Lymphocyte Maturation Factor 40 KDa Subunit; NK Cell Stimulatory Factor Chain; Interleukin-12 Beta Chain; IL-12 Subunit P40; CLMF P40; NKSF2; Natural Killer Cell Stimulatory Factor; IL12; Subunit P40; Interleukin 12; CLMF2; IMD29; NKSF; P40
Reactivity Human
Relevance Monoclonal antibody which binds p40 subunit of interleukin-12.

Background and Research Application
IL-12 is a heterodimeric cytokine comprised of p35 and p40 subunits. IL-12 plays a central role in cell-mediated immunity, promoting the differentiation of CD4+ T cells to the Th1 subset and of CD8+ T cells into mature cytotoxic T lymphocytes (CTLs). Interleukin 12 (IL-12) is produced by dendritic cells, macrophages, neutrophils, and human B-lymphoblastoid cells in response to antigenic stimulation. IL-12 is a potent stimulator of Natural Killer cells. IL-12 therapy has been suggested as a method of enhancing cytocidal anti-tumour immune responses.
This antibody recognises the p40 subunit of human IL-12 and binds to both the free subunit and the p35/p40 heterodimer. It reacts with both stimulated and unstimulated monocytes in immunohistology.
Host Mouse
Immunogen Recombinant human IL-12 produced in Baculovirus.
Immunogen UniProt ID P29459
Subclass IgG1
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 5% FCS.
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Concentration
1mg/ml as standard
Research Area Immunology

References

There are 8 reference entries for this reagent.

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References: 8 entries

Krenkel et al. 2019. Cells. 8(5):. PMID: 31137713.

Mohamed et al. 2019. SAGE Open Med. 7:2050312119828260. PMID: 30783524.

Nagashima et al. 2008. Anticancer Res. 28(2B):1277-83. PMID: 18505066.

Prognostic significance of the local expression of interleukin-12 in patients with advanced gastric cancer.

Europe PMC ID: 18505066

Nakayama et al. 2004. Anticancer Res. 24(5C):3289-94. PMID: 15515423.

Relationships between local and systemic expression of interleukin-12 and plasma levels of vascular endothelial growth factor in patients with gastric cancer.

Europe PMC ID: 15515423

Carter et al. 1997. Hybridoma. 16(4):363-9. PMID: 9309427.

Production and characterization of monoclonal antibodies to human interleukin-12.

Europe PMC ID: 9309427


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References: 8 entries

Krenkel et al. 2019. Cells. 8(5):. PMID: 31137713.

Mohamed et al. 2019. SAGE Open Med. 7:2050312119828260. PMID: 30783524.

Nagashima et al. 2008. Anticancer Res. 28(2B):1277-83. PMID: 18505066.

Prognostic significance of the local expression of interleukin-12 in patients with advanced gastric cancer.

Nakayama et al. 2004. Anticancer Res. 24(5C):3289-94. PMID: 15515423.

Relationships between local and systemic expression of interleukin-12 and plasma levels of vascular endothelial growth factor in patients with gastric cancer.

Carter et al. 1997. Hybridoma. 16(4):363-9. PMID: 9309427.

Production and characterization of monoclonal antibodies to human interleukin-12.


Add a reference

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