Anti-IL12 [1-1A4]
Invented at University of Leeds
- Datasheet
- References (6)
- Inventor Info
Info
| Catalogue Number | 151022 |
| Applications | ELISA IHC RIA WB |
| Antigen/Gene or Protein Targets | Interleukin-12 (IL12) p40 subunit |
| Synonyms | Interleukin 12; Cytotoxic Lymphocyte Maturation Factor 40 KDa Subunit; NK Cell Stimulatory Factor Chain; Interleukin-12 Beta Chain; IL-12 Subunit P40; CLMF P40; NKSF2; Natural Killer Cell Stimulatory Factor; IL12; Subunit P40; Interleukin 12; CLMF2; IMD29; NKSF; P40 |
| Reactivity | Human |
| Relevance |
Monoclonal antibody which binds p40 subunit of interleukin-12. Background and Research Application IL-12 is a heterodimeric cytokine comprised of p35 and p40 subunits. IL-12 plays a central role in cell-mediated immunity, promoting the differentiation of CD4+ T cells to the Th1 subset and of CD8+ T cells into mature cytotoxic T lymphocytes (CTLs). Interleukin 12 (IL-12) is produced by dendritic cells, macrophages, neutrophils, and human B-lymphoblastoid cells in response to antigenic stimulation. IL-12 is a potent stimulator of Natural Killer cells. IL-12 therapy has been suggested as a method of enhancing cytocidal anti-tumour immune responses. This antibody recognises the p40 subunit of human IL-12 and binds to both the free subunit and the p35/p40 heterodimer. It reacts with both stimulated and unstimulated monocytes in immunohistology. |
| Host | Mouse |
| Immunogen | Recombinant human IL-12 produced in Baculovirus. |
| Immunogen UniProt ID | P29459 |
| Subclass | IgG1 |
| Myeloma Used | Sp2/0-Ag14 |
| Recommended Growing Conditions | DMEM + 5% FCS. |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Concentration 1mg/ml as standard |
| Research Area | Immunology |
References: 6 entries
Nagashima et al. 2008. Anticancer Res. 28(2B):1277-83. PMID: 18505066.
IHC
Prognostic significance of the local expression of interleukin-12 in patients with advanced gastric cancer.
Europe PMC ID: 18505066
Nakayama et al. 2004. Anticancer Res. 24(5C):3289-94. PMID: 15515423.
IHC
Relationships between local and systemic expression of interleukin-12 and plasma levels of vascular endothelial growth factor in patients with gastric cancer.
Europe PMC ID: 15515423
Carter et al. 1997. Hybridoma. 16(4):363-9. PMID: 9309427.
Production and characterization of monoclonal antibodies to human interleukin-12.
Europe PMC ID: 9309427
Add a reference
References: 6 entries
Nagashima et al. 2008. Anticancer Res. 28(2B):1277-83. PMID: 18505066.
IHC
Prognostic significance of the local expression of interleukin-12 in patients with advanced gastric cancer.
Nakayama et al. 2004. Anticancer Res. 24(5C):3289-94. PMID: 15515423.
IHC
Relationships between local and systemic expression of interleukin-12 and plasma levels of vascular endothelial growth factor in patients with gastric cancer.
Carter et al. 1997. Hybridoma. 16(4):363-9. PMID: 9309427.
Production and characterization of monoclonal antibodies to human interleukin-12.
Add a reference
