Anti-CyclinB1 [V152]
Invented by Julian Gannon from The Francis Crick Institute
Invented at Cancer Research UK London Research Institute: Clare Hall Laboratories
- Datasheet
- References (8)
- Inventor Info
Info
| Catalogue Number | 151021 |
| Applications | FACS IHC WB |
| Antigen/Gene or Protein Targets | Cyclin B1 |
| Synonyms | CCNB1; Cyclin B1; G2/Mitotic-Specific Cyclin B1; CCNB |
| Reactivity | Mammalian |
| Relevance | Cyclins bind to and regulate the activity of the Cyclin Dependent Protein Kinases (CDKs). Cyclin B1 is a marker of cell proliferation and a key component of the cell cycle progression machinery. Cyclin B1 may prove to be a prognostic marker in neoplasia. |
| Host | Mouse |
| Immunogen | His tagged Hamster Cyclin B1 expressed in bacteria, harvested from inclusion bodies, extracted with 6M guanidine HC1 and purified on Nickel beads. |
| Subclass | IgG1 |
| Myeloma Used | Sp2/0-Ag14 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Notes | Recommended for the detection of cyclin B1 in formalin fixed tissues. |
| Research Area | Cancer, Cell Cycle, Epigenetics & Nuclear Signalling |
References: 8 entries
Gurden et al. 2010. J Cell Sci. 123(Pt 3):321-30. PMID: 20053638.
Cdc20 is required for the post-anaphase, KEN-dependent degradation of centromere protein F.
Europe PMC ID: 20053638
Geley et al. 2001. J Cell Biol. 153(1):137-48. PMID: 11285280.
Anaphase-promoting complex/cyclosome-dependent proteolysis of human cyclin A starts at the beginning of mitosis and is not subject to the spindle assembly checkpoint.
Europe PMC ID: 11285280
Characterization of adriamycin-induced G2 arrest and its abrogation by caffeine in FL-amnion cells with or without p53.
Europe PMC ID: 11120603
Minemoto et al. 2001. Exp Cell Res. 262(1):37-48. PMID: 11120603.
Brandeis et al. 1998. Proc Natl Acad Sci U S A. 95(8):4344-9. PMID: 9539739.
Cyclin B2-null mice develop normally and are fertile whereas cyclin B1-null mice die in utero.
Europe PMC ID: 9539739
Add a reference
References: 8 entries
Gurden et al. 2010. J Cell Sci. 123(Pt 3):321-30. PMID: 20053638.
Cdc20 is required for the post-anaphase, KEN-dependent degradation of centromere protein F.
Geley et al. 2001. J Cell Biol. 153(1):137-48. PMID: 11285280.
Anaphase-promoting complex/cyclosome-dependent proteolysis of human cyclin A starts at the beginning of mitosis and is not subject to the spindle assembly checkpoint.
Characterization of adriamycin-induced G2 arrest and its abrogation by caffeine in FL-amnion cells with or without p53.
Minemoto et al. 2001. Exp Cell Res. 262(1):37-48. PMID: 11120603.
Brandeis et al. 1998. Proc Natl Acad Sci U S A. 95(8):4344-9. PMID: 9539739.
Cyclin B2-null mice develop normally and are fertile whereas cyclin B1-null mice die in utero.
Add a reference
