MCF7 AREc32 Cell Line
Invented by Prof Roland Wolf from University of Dundee
Invented at University of Dundee
- Datasheet
- References (20)
- Inventor Info
Info
| Catalogue Number | 151014 |
| Antigen/Gene or Protein Targets | Antioxidant Response Element (ARE)- Luciferase |
| Parental Line | MCF7 |
| Host | Human |
| Tissue | Breast |
| Disease Keywords | Cancer; drug metabolism |
| Model | Reporter |
| Relevance |
Cell line used to investigate ARE gene expression. Background and Research Application The stable human mammary MCF7-derived reporter cell line called AREc32 contains copies of the rat GST antioxidant response element (ARE) linked to a luciferase gene, such that the induction of the ARE results in luciferase activity. ARE is a transcriptional cis-regulatory element involved in the activation of genes coding for a number of antioxidant proteins and enzymes that work in concert to protect tissues from oxidative insults. This cell line can be used to investigate whether anti-cancer drugs can induce ARE-driven gene expression. |
| Production Details | The ARE-luciferase reporter plasmid was generated using the pGL3-promoter vector containing an SV40 promoter upstream of the firefly luciferase gene. They differ in the number of copies of ARE sequences that have been inserted, in head-to-tail orientation, through Nhe I & Xho I restrictionsites upstream of the promoter-luc+ transcriptional unit. A plasmid was made containing eight copies of the ARE (5'-GTGACAAAGCA-3', with the minimal functional sequence underlined) present in both rat GSTA2 and mouse gsta1; called pGL-8xARE. A linker with the sequence of 5'-CCC-3' and 5'-GGG-3' on the opposite strand was placed between individual cis-elements.pGL-8xARE, was stably transfected into MCF7 cells using the calcium phosphate method. Transfected cells were selected using 0.8 mg/mL G418 in the media for 3 to 4 weeks. The G418-resistant clones were isolated and screened by measuring their basal and inducible (obtained by treatment with 50 Amol/L t-BHQ) luciferase activities as described above. Positive clones, which showed low background and high inducible luciferase activity, were passaged and maintained in growth medium containing 0.8 mg/mL G418. |
| Research Area | Cancer, Drug Discovery & Development |
| Recommended Growing Conditions | DMEM with glutamax supplemented with 10% fetal bovine serum and antibiotics. Do not culture beyond 15 passages after revival. |
| Notes |
Concentration Vial has between 1-5 million cells as standard, however this may vary. |
| Cellosaurus ID | CVCL_1D32 |
References: 20 entries
Gameiro et al. 2017. Sci Rep. 7:45701. PMID: 28361919.
Discovery of the first dual GSK3β inhibitor/Nrf2 inducer. A new multitarget therapeutic strategy for Alzheimer's disease.
Europe PMC ID: 28361919
MacLeod et al. 2016. Br J Cancer. :. PMID: 27824809.
Aldo-keto reductases are biomarkers of NRF2 activity and are co-ordinately overexpressed in non-small cell lung cancer.
Europe PMC ID: 27824809
Basar et al. 2016. Phytochem Anal. 27(5):233-8. PMID: 27527356.
Utilization of the Ability to Induce Activation of the Nuclear Factor (Erythroid-derived 2)-like Factor 2 (Nrf2) to Assess Potential Cancer Chemopreventive Activity of Liquorice Samples.
Europe PMC ID: 27527356
Bräunig et al. 2016. Chemosphere. 156:181-90. PMID: 27176940.
Bioanalytical effect-balance model to determine the bioavailability of organic contaminants in sediments affected by black and natural carbon.
Europe PMC ID: 27176940
Brack et al. 2016. Sci Total Environ. 544:1073-118. PMID: 26779957.
Effect-directed analysis supporting monitoring of aquatic environments--An in-depth overview.
Europe PMC ID: 26779957
New melatonin-cinnamate hybrids as multi-target drugs for neurodegenerative diseases: Nrf2-induction, antioxidant effect and neuroprotection.
Europe PMC ID: 26496465
Rücker et al. 2015. Org Biomol Chem. 13(10):3040-7. PMID: 25622264.
Buendia et al. 2015. Future Med Chem. 7(15):1961-9. PMID: 26496465.
Enhancing the anti-inflammatory activity of chalcones by tuning the Michael acceptor site.
Europe PMC ID: 25622264
Escher et al. 2012. J Environ Monit. 14(11):2877-85. PMID: 23032559.
Water quality assessment using the AREc32 reporter gene assay indicative of the oxidative stress response pathway.
Europe PMC ID: 23032559
Wang et al. 2007. Proc Natl Acad Sci U S A. 104(49):19589-94. PMID: 18048326.
Identification of retinoic acid as an inhibitor of transcription factor Nrf2 through activation of retinoic acid receptor alpha.
Europe PMC ID: 18048326
Wang et al. 2006. Cancer Res. 66(22):10983-94. PMID: 17108137.
Generation of a stable antioxidant response element-driven reporter gene cell line and its use to show redox-dependent activation of nrf2 by cancer chemotherapeutic agents.
Europe PMC ID: 17108137
Add a reference
References: 20 entries
Gameiro et al. 2017. Sci Rep. 7:45701. PMID: 28361919.
Discovery of the first dual GSK3β inhibitor/Nrf2 inducer. A new multitarget therapeutic strategy for Alzheimer's disease.
MacLeod et al. 2016. Br J Cancer. :. PMID: 27824809.
Aldo-keto reductases are biomarkers of NRF2 activity and are co-ordinately overexpressed in non-small cell lung cancer.
Basar et al. 2016. Phytochem Anal. 27(5):233-8. PMID: 27527356.
Utilization of the Ability to Induce Activation of the Nuclear Factor (Erythroid-derived 2)-like Factor 2 (Nrf2) to Assess Potential Cancer Chemopreventive Activity of Liquorice Samples.
Bräunig et al. 2016. Chemosphere. 156:181-90. PMID: 27176940.
Bioanalytical effect-balance model to determine the bioavailability of organic contaminants in sediments affected by black and natural carbon.
Brack et al. 2016. Sci Total Environ. 544:1073-118. PMID: 26779957.
Effect-directed analysis supporting monitoring of aquatic environments--An in-depth overview.
New melatonin-cinnamate hybrids as multi-target drugs for neurodegenerative diseases: Nrf2-induction, antioxidant effect and neuroprotection.
Rücker et al. 2015. Org Biomol Chem. 13(10):3040-7. PMID: 25622264.
Buendia et al. 2015. Future Med Chem. 7(15):1961-9. PMID: 26496465.
Enhancing the anti-inflammatory activity of chalcones by tuning the Michael acceptor site.
Escher et al. 2012. J Environ Monit. 14(11):2877-85. PMID: 23032559.
Water quality assessment using the AREc32 reporter gene assay indicative of the oxidative stress response pathway.
Wang et al. 2007. Proc Natl Acad Sci U S A. 104(49):19589-94. PMID: 18048326.
Identification of retinoic acid as an inhibitor of transcription factor Nrf2 through activation of retinoic acid receptor alpha.
Wang et al. 2006. Cancer Res. 66(22):10983-94. PMID: 17108137.
Generation of a stable antioxidant response element-driven reporter gene cell line and its use to show redox-dependent activation of nrf2 by cancer chemotherapeutic agents.
Add a reference
Inventor Information
Inventors
|
Prof Roland Wolf |
