A:
Most antibodies are quite robust and should retain functional activity if kept refrigerated at 2—8oC for up to 12 months.
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Monoclonal Antibodies
This section contains frequently asked questions on the production, supply, usage and storage of monoclonal antibodies. If you cant find the answer you need please use the "suggest an FAQ" button to submit your question, which will ultimately benefit the life sciences community.
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Monoclonal Antibodies
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suggest an FAQQ: How are the antibodies stored and dispatched?
A:
They are stored vialled at -20°C, and dispatched at ambient temperature in padded postage bags containing cool packs.
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Q: Will the antibodies work in Western Blots?
A:
This depends on whether the target antigen is denatured after electrophoresis.
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Q: Are there any recommended dilutions for usage?
A:
These depend on the intended application and must be determined individually.
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Q: How is the antibody concentration determined?
A:
By uv absorption at 280 nm, in a 1 cm path length cuvette using an extinction coefficient of 1.4
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Q: How are the monoclonal antibodies purified?
A:
IgG by Protein A (murine) or Protein G (rat) affinity chromatography
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Q: Are bovine serum supplements used?
A:
Yes, from USDA approved serum suppliers
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Q: How are the monoclonal antibodies produced?
A:
By in vitro production methods; we do not produce in ascites fluid.
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Q: Can monoclonal antibodies be supplied in other formulations such as, “bulk”size or without sodium azide?
A:
Not off the shelf, but can be considered as a one-off custom order.
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Q: How are monoclonal antibodies supplied?
A:
As 1.1 ml aliquots (to include overage) at a concentration of 0.9--1.0 mg/ml
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