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Info
| Catalogue Number | 158059 |
| Applications | IHC WB |
| Antigen/Gene or Protein Targets | Major histocompatibility complex class 2 |
| Reactivity | Salmonids |
| Relevance |
MHC class II molecules are heterodimeric glycoproteins composed of a α and a β chain, which are constitutively displayed at the surface of professional antigen-presenting cells (APCs) such as dendritic cells (DCs), macrophages, and B cells. MHC class II molecules present antigens of exogenous origin to CD4+ T cells. This is a marker for professional antigen-presenting cells. |
| Host | Rabbit |
| Immunogen | Ovalbumin-conjugated synthetic peptide. |
| Formulation | Unpurified anti-serum from rabbit preserved in 0.02% Thiomersal |
| Molecular Weight (kDa) | 26 |
| Notes |
Applications and Recommended Usage Conditions: - WB recommended to be used at 1/100 dilution. Use trout spleen lysates as positive control. - IHC recommended to be used at 1/100 dilution. Antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0. - Publication - Veenstra et al. 2019. Fish Shellfish Immunol. 87:559- 564. PMID: 30731214. Unpurified anti-serum from rabbit preserved in 0.02% Thiomersal |
| Positive Control | WB: Trout spleen lysates IHC: Formalin-fixed, paraffin-embedded rainbow-trout adipose tissues |
| Research Area | Cell Type or Organelle Marker, Immunology |
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Inventor Information
Inventors
|
Abdo Alnabulsi |
