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MCF10A-HER2
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Catalogue Number 157986
Antigen/Gene or Protein Targets Human Epidermal Receptor 2 (HER2/EBB2/neu), overexpressed in 25% breast cancers, treatments include Trastuzumab and Lapatinib. This plasmid also contains GFP for selection and tracking purposes.
Parental Line MCF10A
Tissue Breast
Disease Keywords Breast cancer
Model Immortalised Line
Relevance Expressed at low levels in normal breast tissues but overexpressed in a subset of breast and ovarian cancers.
Production Details Inducible HER2 construct was stably transduced in MCF10A cell line using a third generation lentiviral transduction system.
Conditional Yes
Conditional Description Conditional HER2 expression under tetracyline response element (TRE) regulated by the addition of doxycyline in breast epithelial cell line (MCF10A).
Research Area Cancer
Positive Control Any other HER2 cell line such as BT474 may be used as a positive control.
Notes The GFP is in the same construct as the inducible HER2 but both genes are regulated by two different promoters. GFP expression is under the control of a weak EF1α promoter whereas the HER2 is induced by a strong TRE promoter.

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