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Catalogue Number
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157927 |
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Antigen/Gene or Protein Targets
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Branched-galactan
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Synonyms
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β-1,6-galactosyl substitution of β-1,4-galactan requiring more than three backbone residues for optimized recognition.
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Relevance
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The mechanistic basis of primary cell wall heterogeneity in the context of cell type differentiation and cell function is a poorly understood factor in plant development. Plant cell walls are cellulosic composites that underpin and control many aspects of plant cell and organ growth by virtue of the presence of adherent cell surfaces that ramify throughout developing and mature organs. In addition to cellulosic fibers that provide structural strength to cell walls, several sets of noncellulosic matrix polysaccharides are present (Burton et al., 2010; Doblin et al., 2010). These are often structurally hypervariable and are important factors in cell wall biogenesis, cell extension, and cell function. Not all cell surfaces are the same in terms of precise structures of individual polysaccharides, and cell-wall glycan molecular configurations display developmental dynamics and cell type specificities (Burton et al., 2010; Knox 2008; Lee et al., 2012; Torode et al., 2016).
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Host
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Rat
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Immunogen
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Lime pectin with a degree of methyl-esterification (DE) of 22.9%, a degree of amidation of 27.3% and an average molecular mass of 84 kDa.
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Positive Control
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IgG
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Myeloma Used
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IR983F
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Notes
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The antibody was isolated from a wide screen of cell lines obtained subsequent to immunization with cell wall polysaccharides. Glycan microarrays indicated that LM26 recognises a (1-6)-galactosyl substitution in (1-4)-beta-D-galactans and that it has no recognition of linear (1-4)-beta-D-galactan. LM26 can recognise pectic polysaccharides in several species and in a wide range of plant organs the LM26 epitope can be specifically detected in cell walls of phloem sieve elements.
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Research Area
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Plant Science
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