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HeLa FRT/TO (YFP-Astrin Δ70 siRNA res) cell line
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Invented by Duccio Conti, at Queen Mary University of London Viji M. Draviam at Queen Mary University of London

Info

Catalogue Number 157865
Parental Line HeLa cell line
Synonyms HeLa FRT/TO YFP-Astrin WT cell line conditionally expressing siRNA-resistant YFP-Astrin WT.
Host Human
Tissue Cervix
Model Cancer Model
Production Details HeLa FRT/TO YFP-Astrin Δ70 cell line conditionally expressing siRNA-resistant YFP-Astrin Δ70.

Created by transfection of HeLa Flp recombinase cell line with pCDNA5-FRT/TO-YFP-Astrin Δ70 expression plasmid, followed by a brief Hygromycin selection and sorting for YFP positive using FACS.
Research Area Cell Type or Organelle Marker, Cell Structure and Motility, Cell Signaling & Signal Transduction, Cell Cycle, Cancer
Notes siRNA oligos to target Astrin mRNA 5’ UTR (GACUUGGUCUGAGACGUGAtt) or Astrin 52 oligo (UCCCGACAACUCACAGAGAAAUU).
Astrain Δ70 mutant was generated by PCR amplification of 1–1122 coding region of Astrin and subcloning into the desired expression vector.

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Inventor Information

Inventors

Duccio Conti

Duccio Conti

Viji M. Draviam

Viji M. Draviam

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