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E. coli Strain SQ110/ΔlptD MG1655 bacterial culture

Info

Catalogue Number 156499
Antigen/Gene or Protein Targets rRNA operons; lptD
Bacterial Resistance N/A
Relevance The Mankin lab at UIC has engineered two E. coli strains, (SQ110/ΔtolC and SQ110/ΔlptD), that are particularly suitable for selection of resistant mutants to bacterial protein synthesis inhibitors.
Protein synthesis inhibitors can be detected in natural extracts by metabolic labeling or in vitro translation. Identifying the target site of these inhibitors can provide foremost insights regarding their mode of action or chemical nature. Localizing a resistant mutation in the ribosome is an excellent strategy to identify the binding site of an inhibitor.

To enable this process, SQ110/ΔtolC and SQ110/ΔlptD lack 6 out of 7 endogenous rRNA operons. The benefits of this are two-fold: hyper-sensitivity to protein synthesis inhibition, and ease of characterization of resistant mutants. The two strains complement one another by their differential inactivation of tolC and expression of a truncated lptD gene, which lowers the minimum inhibitory concentrations (MICs) or enhances cell permeability to drugs.
Genomic Feature Deletion of six out of seven rRNA operons. ΔlptD::lptD(Asp330-Asp352 deletion)+cat-3'
Research Area Drug Discovery & Development, Bacteriology

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References: 1 entry

Orelle et al. 2013. Antimicrob Agents Chemother. 57(12):5994-6004. PMID: 24041905.


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References: 1 entry

Orelle et al. 2013. Antimicrob Agents Chemother. 57(12):5994-6004. PMID: 24041905.


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