HEK293T BRE-Luc/TK Renilla cell line

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Catalogue Number	156440
Antigen/Gene or Protein Targets	Bone morphogenetic protein (BMP) signalling
Parental Line	HEK293T cell line
Host	Human
Tissue	Kidney
Model	Reporter
Relevance	The human embryo kidney cell line HEK293T modified for use as a reporter cell line for bone morphogenetic protein (BMP) signalling. The cell line stably expresses BMP responsive element (BRE)-Luciferase. The cell line also contains a Renilla reporter driven by the thymidine kinase (TK) promoter to act as an internal control. BMP is a transforming growth factor β (TGF-β) superfamily member.
Production Details	Made by transfecting the human embryo kidney cell line HEK293T with BRE-Luciferase plasmid, TK Renilla plasmid and pRetroSuper for puromycin resistance. Pool
Conditional Description	Luciferase is expressed in the presence of bone morphogenetic protein (BMP) signalling
Research Area	Cancer, Cell Signaling & Signal Transduction, Developmental Biology
Recommended Growing Conditions	Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal calf serum (FCS)
Notes	Unpublished

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Caroline Hill

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	The Francis Crick Institute
	14 reagents listed on Ximbio

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