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HEK293T BRE-Luc/TK Renilla cell line

Invented by Caroline Hill at The Francis Crick Institute

Info

Catalogue Number 156440
Antigen/Gene or Protein Targets Bone morphogenetic protein (BMP) signalling
Parental Line HEK293T cell line
Host Human
Tissue Kidney
Model Reporter
Relevance The human embryo kidney cell line HEK293T modified for use as a reporter cell line for bone morphogenetic protein (BMP) signalling. The cell line stably expresses BMP responsive element (BRE)-Luciferase. The cell line also contains a Renilla reporter driven by the thymidine kinase (TK) promoter to act as an internal control. BMP is a transforming growth factor β (TGF-β) superfamily member.
Production Details Made by transfecting the human embryo kidney cell line HEK293T with BRE-Luciferase plasmid, TK Renilla plasmid and pRetroSuper for puromycin resistance.

Pool
Conditional Description Luciferase is expressed in the presence of bone morphogenetic protein (BMP) signalling
Research Area Cancer, Cell Signaling & Signal Transduction, Developmental Biology
Recommended Growing Conditions Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal calf serum (FCS)
Notes Unpublished

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