| Catalogue Number | 156439 |
| Antigen/Gene or Protein Targets | Bone morphogenetic protein (BMP) signalling |
| Parental Line | MDA-MB-231 cell line |
| Host | Human |
| Tissue | Breast |
| Disease Keywords | Breast cancer |
| Model | Reporter |
| Relevance | The human breast cancer cell line MDA-MB-31 modified for use as a reporter cell line for bone morphogenetic protein (BMP) signalling. The cell line stably expresses BMP responsive element (BRE)-Luciferase. The cell line also contains a Renilla reporter driven by the thymidine kinase (TK) promoter to act as an internal control. BMP is a transforming growth factor β (TGF-β) superfamily member. |
| Production Details |
Made by transfecting the breast cancer cell line MDA-MB-31 with BRE-Luciferase plasmid, TK Renilla plasmid and pRetroSuper for puromycin resistance. Clonal |
| Conditional Description | Luciferase is expressed in the presence of bone morphogenetic protein (BMP) signalling |
| Research Area | Cancer, Cell Signaling & Signal Transduction, Developmental Biology |
| Recommended Growing Conditions | Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal calf serum (FCS)+ 50 µg/ml blasticidin |
Invented by Caroline Hill at The Francis Crick Institute
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Caroline Hill |
