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MDA-MB-231 BRE-Luc/TK Renilla cell line

Invented by Caroline Hill at The Francis Crick Institute

Info

Catalogue Number 156439
Antigen/Gene or Protein Targets Bone morphogenetic protein (BMP) signalling
Parental Line MDA-MB-231 cell line
Host Human
Tissue Breast
Disease Keywords Breast cancer
Model Reporter
Relevance The human breast cancer cell line MDA-MB-31 modified for use as a reporter cell line for bone morphogenetic protein (BMP) signalling. The cell line stably expresses BMP responsive element (BRE)-Luciferase. The cell line also contains a Renilla reporter driven by the thymidine kinase (TK) promoter to act as an internal control. BMP is a transforming growth factor β (TGF-β) superfamily member.
Production Details Made by transfecting the breast cancer cell line MDA-MB-31 with BRE-Luciferase plasmid, TK Renilla plasmid and pRetroSuper for puromycin resistance.

Clonal
Conditional Description Luciferase is expressed in the presence of bone morphogenetic protein (BMP) signalling
Research Area Cancer, Cell Signaling & Signal Transduction, Developmental Biology
Recommended Growing Conditions Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal calf serum (FCS)+ 50 µg/ml blasticidin

References

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References: 1 entry

Grönroos et al. 2012. Mol Cell Biol. 32(14):2904-16. PMID: 22615489.


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References: 1 entry

Grönroos et al. 2012. Mol Cell Biol. 32(14):2904-16. PMID: 22615489.


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Inventor Information