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Anti-GST scFv recombinant antibody (HRP)

Info

Catalogue Number 154802
Applications ELISA WB DB
Antigen/Gene or Protein Targets GST
Synonyms Glutathione S-Transferase
Reactivity N/A
Relevance GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.

Functions as a primary and secondary antibody in one.
Immunogen GST antigen
Formulation PBS (0.1M) + 0.5M imidazole at pH 7.4
This product was purified using affinity chromatography (nickel resin)
Concentration 1mg/ml
Molecular Weight (kDa) Various
Research Area Epitope Tags
Notes This product is a recombinant single chain variable fragment (scFv) fused to Horseradish peroxidase (HRP) and produced in N. Benthamiana plants.
Volume: 100ul
Storage: 40C
Form & colour: Reddish/brown liquid
Stability: Up to 1 year from delivery
Conjugation notes: N-terminal conjugated HRP
Clonality: Monoclonal
Host species: Made in N. Benthamiana plants

Western blot: Suggested working dilution 1/1000-1/5000 to detect 1ug antigen in 5% milk. Suggested incubation condition, 1hr at 370C/40C overnight
ELISA: Suggested working dilution 1/5000-1/10000. Suggested incubations conditions, 1hr at 370C
Dot blot: Use at an assay dependent concentration

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