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Anti-Integrin β-1D (CD29) [2B1] monoclonal antibody
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Invented by Prof Arnoud Sonnenberg at Netherlands Cancer Institute

Info

Catalogue Number 154743
Applications IHC WB
Antigen/Gene or Protein Targets Integrin β1D
Synonyms ITGB1; MSK12
Reactivity Dog, Human, Mouse and Pig
Relevance ITGB1 is a cell surface receptor that in humans is encoded by the ITGB1 gene. This integrin associates with integrin alpha 1 and integrin alpha 2 to form integrin complexes which function as collagen receptors. It also forms dimers with integrin alpha 3 to form integrin receptors for netrin 1 and reelin. These and other integrin beta 1 complexes have been historically known as very late activation (VLA) antigens.
Integrin beta 1 is expressed as at least four different isoforms. In cardiac muscle and skeletal muscle, the integrin beta-1D isoform is specifically expressed, and localizes to costameres, where it aids in the lateral force transmission from the Z-discs to the extracellular matrix. Abnormal levels of integrin beta-1D have been found in limb girdle muscular dystrophy and polyneuropathy
Host Mouse
Immunogen A mouse was immunized with a synthetic peptide corresponding to the C-terminal 24 amino acids of integrin â1D including an appending N-terminal cysteine (CQENPIYKSPINNFKNPNYGRKAGL) coupled to keyhole limpet hemocyanin.
Subclass IgG1
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions RPMI +10% FBS ultra low IgG, 37 ⁰C 5% CO2
Strain Balb/c
Notes Fusion partners: SP2/0 mouse myeloma cells with spleen cells from a BALB/c mouse. Recognizes specifically the cytoplasmic domain of integrin subunit β1D present in cardiac and skeletal muscle.
Research Area Adhesion, Cell Signaling & Signal Transduction

References

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References: 1 entry

van der Flier et al. 1997. Dev Dyn. 210(4):472-86. PMID: 9415431.


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References: 1 entry

van der Flier et al. 1997. Dev Dyn. 210(4):472-86. PMID: 9415431.


Add a reference