Anti-Integrin β-1D (CD29) [Beta1G2] monoclonal antibody
Invented by Prof Arnoud Sonnenberg from Netherlands Cancer Institute
Invented at Netherlands Cancer Institute
- Datasheet
- References (1)
- Inventor Info
Info
| Catalogue Number | 154741 |
| Applications | IHC WB |
| Antigen/Gene or Protein Targets | Integrin β1D |
| Synonyms | ITGB1; MSK12 |
| Reactivity | Human and Mouse |
| Relevance |
ITGB1 is a cell surface receptor that in humans is encoded by the ITGB1 gene. This integrin associates with integrin alpha 1 and integrin alpha 2 to form integrin complexes which function as collagen receptors. It also forms dimers with integrin alpha 3 to form integrin receptors for netrin 1 and reelin. These and other integrin beta 1 complexes have been historically known as very late activation (VLA) antigens. Integrin beta 1 is expressed as at least four different isoforms. In cardiac muscle and skeletal muscle, the integrin beta-1D isoform is specifically expressed, and localizes to costameres, where it aids in the lateral force transmission from the Z-discs to the extracellular matrix. Abnormal levels of integrin beta-1D have been found in limb girdle muscular dystrophy and polyneuropathy |
| Host | Mouse |
| Immunogen | A mouse was immunized with a synthetic peptide corresponding to the C-terminal 24 amino acids of integrin β1D including an appending N-terminal cysteine (CQENPIYKSPINNFKNPNYGRKAGL) coupled to keyhole limpet hemocyanin. |
| Subclass | IgG2a |
| Myeloma Used | Sp2/0-Ag14 |
| Recommended Growing Conditions | DMEM + 10% FBS ulta low IgG, 37 ⁰C 5% CO2 |
| Strain | Balb/c |
| Notes | Fusion partners: SP2/0 mouse myeloma cells with spleen cells from a BALB/c mouse. Recognizes specifically the cytoplasmic domain of integrin subunit β1D present in cardiac and skeletal muscle. |
| Research Area | Adhesion, Cell Signaling & Signal Transduction |
