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Anti-Mycobacterial 65-kDa hsp [Nd4]

Invented by Professor Pranab K Das

Info

Catalogue Number 154069
Applications ELISA IHC WB
Antigen/Gene or Protein Targets Mycobacterial 65-kDa hsp
Reactivity Mycobacterium bovis and Mycobacterium avium
Relevance The 65 kDa protein from M.tuberculosis and M.leprae contains a striking degree of sequence homology, differing in only 28 amino acids. This antibody can distinguish the two making it potentially useful in the serodiagnosis of tuberculosis and leprosy. In addition the C-terminal epitope of mycobacterial 65-kDa hsp is shared with human epidermal suprabasal cytokeratin 1/2 making this antibody a marker of cytokeratin 1/2.
Host Mouse
Immunogen 65-kDa antigen isolated from M.tuberculosis (RIVM-strain 7114)
Positive Control M.tuberculosis
Subclass IgG1
Myeloma Used P3X63Ag8.653
Recommended Growing Conditions RPMI 1640
Strain Balb/c
Notes Reacts with M. bovis BCG, M.tuberculosis complex and M.avium complex. Does not react with 65 kDa protein from M.leprae. Epitope on mycobacterial 65 kDa heat shock protein mapped to residues in the carboxy-terminal region of amino acid residues 525-540
Research Area Bacteriology, Immunology, Microbiology

References

There are 4 reference entries for this reagent.

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References: 4 entries

Rambukkana et al. 1992. Immunology. 77(2):267-76. PMID: 1385316.

Mycobacterial 65,000 MW heat-shock protein shares a carboxy-terminal epitope with human epidermal cytokeratin 1/2.

Europe PMC ID: 1385316

Rambukkana et al. 1991. FEMS Microbiol Immunol. 3(1):39-45. PMID: 1711877.

Identification of a novel B-cell epitope of restricted specificity on the hsp 65-kDa protein of Mycobacterium tuberculosis.

Europe PMC ID: 1711877


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References: 4 entries

Rambukkana et al. 1992. Immunology. 77(2):267-76. PMID: 1385316.

Mycobacterial 65,000 MW heat-shock protein shares a carboxy-terminal epitope with human epidermal cytokeratin 1/2.

Rambukkana et al. 1991. FEMS Microbiol Immunol. 3(1):39-45. PMID: 1711877.

Identification of a novel B-cell epitope of restricted specificity on the hsp 65-kDa protein of Mycobacterium tuberculosis.


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Inventor Information

Inventors

Professor Pranab K Das

Professor Pranab K Das

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