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Monoclonal Antibodies

Anti-Myostatin [Myo2/1A]

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Clone Myo 2/1A used to detect Myostatin expression by ICC. Effect of recombinant Mst-113 protein and anti-Mst antibody on myogenesis in differentiating C3H 10T(1/2), assessed by using myogenin immunocytochemical staining. Cells were incubated with medium alone (C, control), graded concentrations of recombinant Mst-113 or anti-Mst antibody (Mst Ab) for 1 wk. A, Immunocytochemical staining using an antibody against myogenin; quantitative image analysis of stained cells is shown in B. Asterisks denote the P values for the statistical comparison of treatment group against medium control. No 1st Ab, Cells not treated with first antibody. Magnification, ×400. Source: Artaza et al. 2005. Endocrinology. 146(8):3547-57. PMID: 15878958.
Clone Myo 2/1A used to detect Myostatin expression by Western Blot with detection at 52 kDa. A: Luminol detection of Western blots with monoclonal antibody against Mst at 3 d after transfection. Source: Artaza et al. 2005. Endocrinology. 146(8):3547-57. PMID: 15878958.
Clone Myo 2/1A used to detect C/EBP α to assess adipogenic differentiation C3H 10T(1/2) by IHC. B, Cells were incubated for 4 d (C/EBPα) and 1 wk (adiponectin) with graded concentrations of recombinant Mst-113 or anti-Mst antibody, and plates were divided for either trypsination and Western blot on the cell extracts (30 μg/lane) with an antibody against C/EBP-α. Source: Artaza et al. 2005. Endocrinology. 146(8):3547-57. PMID: 15878958. 
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Catalogue Number 153655
Applications WB
Antigen/Gene or Protein Targets Myostatin
Synonyms Growth/differentiation factor 8, GDF-8, myostatin, GDF8, MSTN
Reactivity Human
Relevance Myostatin is a well-characterized negative regulator of skeletal muscle and can inhibit myogenesis and stimulate adipogenesis. Clone Myo 2/1A has been shown to have the reverse effect, up-regulate myogenesis and down-regulate adipogenesis.
Host Mouse
Immunogen Recognizes the 113 amino acid carboxy-terminal fragment of Myostatin protein
Positive Control Muscle fibre
Molecular Weight (kDa) 52
Myeloma Used Sp2/0-Ag14
Notes Suggested dilutions for use

Western Blot 1:500
Immunocytochemistry 100 μg/mL

Research Areas: Endocrinology;Cell Signaling & Signal Transduction
Research Area Cell Signaling & Signal Transduction

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References: 2 entries

Artaza et al. 2005. Endocrinology. 146(8):3547-57. PMID: 15878958.

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Myostatin inhibits myogenesis and promotes adipogenesis in C3H 10T(1/2) mesenchymal multipotent cells.

Europe PMC ID: 15878958

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References: 2 entries

Artaza et al. 2005. Endocrinology. 146(8):3547-57. PMID: 15878958.

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Myostatin inhibits myogenesis and promotes adipogenesis in C3H 10T(1/2) mesenchymal multipotent cells.

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