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Streptococcus uberis mutated Bacterial Culture

Invented by Prof James Leigh at University of Nottingham

Info

Catalogue Number 153316
Antigen/Gene or Protein Targets Pragmatic Insertional Mutation Mapping of Streptococcus uberis
Classification Streptococcus uberis strain 0140J
Bacterial Resistance Erythromycin
Synonyms S. uberis, Strep, Streptococci, cocci, coccus, Lactobacillales
Relevance Streptococcus is a genus of coccus Gram-positive bacteria belonging to the lactic acid producing bacterial order Lactobacillales.

These bacteria multiply in chains or pairs as cell division occurs along a single access. Several strains are involved in human pathogenesis and responsible for a plothera of different diseases: pharyngitis, conjunctivitis, meningitis, bacterial pneumonia, endocarditis, erysipelas, and necrotising fasciitis.

However, many streptococcal species are not human pathogens and consist as part of the commensal human microbiota of the skin, mouth, upper respiratory tract and intestine. Streptococcus uberis is associated with intramammary infection and mastitis in ruminants.

The Pragmatic Insertional Mutation Mapping (PIMMS) approach enables detection of essential and conditionally essential genes, as well as sequences known to be associated with basic cellular functions in Streptococci.

PIMMS expands the methodology used to locate insertional mutations in individual mutant bacteria to the analysis of mutations in entire bacterial populations. This technique can also be applied to other types of Streptococci and related bacterial species like Enterococcus.
Conditional No
In vitro applications This technique enables researchers to align bacterial genes with phenotypic changes
Production Details Random insertion mutagenesis by electroporation with sequence element S1 (pGh9:ISS1)
Notes Each tube contains

• 10ul of harvested bacterial mutants in pyrogen-free saline containing 25% (v/v) glycerol

• Number of independent mutants per tube ~ 1-2 x 104 for which all of the mutations present have been mapped

• Total number of bacteria present approximately ~108 / tube

Storage and viability

• Material should be stored frozen

• Material frozen at -80oC retains full viability for several years (at least 10)

Reconstitution of material

• Material may be used directly

• Material may be amplified by dilution in ice-cold Todd Hewitt (TH) broth and plating for single colonies on TH agar (pre-warmed to 37oC) containing erythromycin (1ug/ml). Colonies can be harvested after 24h growth at 37oC and reconstituted as required. For storage, resuspended bacterial cells should be mixed with and equal volume of 50% (v/v) glycerol and frozen at -80oC
Research Area Bacteriology, Gene Expression, Genetic Studies Tools

References: 4 entries

Blanchard et al. 2016. Front Microbiol. 7:1645. PMID: 27826289.

PIMMS (Pragmatic Insertional Mutation Mapping System) Laboratory Methodology a Readily Accessible Tool for Identification of Essential Genes in Streptococcus.

Europe PMC ID: 27826289

Blanchard et al. 2015. Front Genet. 6:139. PMID: 25914720.

Transposon insertion mapping with PIMMS - Pragmatic Insertional Mutation Mapping System.

Europe PMC ID: 25914720


Add a reference

References: 4 entries

Blanchard et al. 2016. Front Microbiol. 7:1645. PMID: 27826289.

PIMMS (Pragmatic Insertional Mutation Mapping System) Laboratory Methodology a Readily Accessible Tool for Identification of Essential Genes in Streptococcus.

Europe PMC ID: 27826289

Blanchard et al. 2015. Front Genet. 6:139. PMID: 25914720.

Transposon insertion mapping with PIMMS - Pragmatic Insertional Mutation Mapping System.

Europe PMC ID: 25914720


Add a reference

References: 4 entries

Blanchard et al. 2016. Front Microbiol. 7:1645. PMID: 27826289.

PIMMS (Pragmatic Insertional Mutation Mapping System) Laboratory Methodology a Readily Accessible Tool for Identification of Essential Genes in Streptococcus.

Blanchard et al. 2015. Front Genet. 6:139. PMID: 25914720.

Transposon insertion mapping with PIMMS - Pragmatic Insertional Mutation Mapping System.


Add a reference


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