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RT112/84 Cell Line

Info

Catalogue Number 153250
Host Human
Tissue Bladder
Disease Keywords Human bladder carcinoma epithelial
Model Tumour line
Relevance A slow growing tumour which does not form colonies in soft agar. Tumorigenic in nude mice, RT1112/84 cells possess a low plating efficiency requiring 400 cells/dish to form any colonies.
Conditional No
Research Area Cancer, Drug Discovery & Development
Growth/Phenotype Keywords Adherent
Recommended Growing Conditions Subculture Routine: Split sub-confluent cultures (70-80%) seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C. Subculture weekly - cells are difficult to trypsinise, and should be kept sub-confluent.Culture Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Notes STR-PCR Data:
Amelogenin: X
CSF1PO: 10,11
D13S317: 13,14
D16S539: 11,13
D5S818: 10,13
D7S820: 11,12
THO1: 7
TPOX: 8,11
vWA: 14,17
Cellosaurus ID CVCL_2714

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