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3.334 SC5/8 Cell Line

Info

Catalogue Number 153249
Parental Line 3T3TK3 cells and normal rat kidney cells
Host Hybrid
Tissue Fibroblast
Model Tumour line
Relevance Hybrid between un-transformed mouse 3T3TK3 cells and normal rat kidney cells transformed with RSV. The hybrid cells express a non-transformed phenotype. The cells exhibit anchorage-independent growth at 35°C but not at 39.5°C, the non-permissive temperature and showed differences in microfilament bundle formation at both temperatures.
Conditional No
Research Area Adhesion, Cell Structure and Motility
Growth/Phenotype Keywords Adherent
Recommended Growing Conditions Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 35°C; 5% CO2.Culture Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Notes Rat x Mouse hybrid
Cellosaurus ID CVCL_7237

References

There are 2 reference entries for this reagent.

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References: 2 entries

Marshall et al. 1980. Exp Cell Res. 127(2):373-84. PMID: 6247175.

Suppression of the transformed phenotype with retention of the viral 'src' gene in cell hybrids between Rous sarcoma virus-transformed rat cells and untransformed mouse cells.

Europe PMC ID: 6247175


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References: 2 entries

Marshall et al. 1980. Exp Cell Res. 127(2):373-84. PMID: 6247175.

Suppression of the transformed phenotype with retention of the viral 'src' gene in cell hybrids between Rous sarcoma virus-transformed rat cells and untransformed mouse cells.


Add a reference

Inventor Information