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Anti-C-NAP1/CEP250 [6F2C8]

Invented by Prof Ciaran Morrison at National University of Ireland, Galway

Info

Catalogue Number 153171
Applications IHC IP WB
Antigen/Gene or Protein Targets Centrosomal Nek2-Associated Protein 1
Synonyms Centrosome-associated protein CEP250; Centrosomal Protein 250; Centrosomal Protein 250kDa; Centrosomal Nek2-Associated Protein 1; 250 KDa Centrosomal Protein; Centrosomal Protein 2; C-NAP1; CNAP1
Reactivity Human
Relevance Centrosomal Protein 250 (CEP250), also known as Centrosomal Nek2-Associated Protein 1 (C-NAP1), is a core centrosomal protein required for centriole-centriole cohesion during interphase of the cell cycle. CEP250 dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome.
Host Mouse
Immunogen Recombinant human C-NAP1 amino acids 1513-1750 (bacterially expressed from pGEX4T as a GST fusion, then thrombin-cleaved and purified).
Subclass IgG1 kappa
Positive Control Immunofluorescence and Western Blot: Jurkat cells, hTERT-RPE1 cells, Immunofluorescence only: PC3 cells, DU145 cells, PWR1E cells, Peripheral Blood Mononuclear Cells (PBMCs)
Molecular Weight (kDa) 250
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 10% FBS + hypoxanthine supplement + non-essential amino acids
Research Area Cell Cycle, Immunology

References: 2 entries

Flanagan et al. 2017. Mol Biol Cell. :. PMID: 28100636.

IHC WB

Centriole splitting caused by loss of the centrosomal linker protein C-NAP1 reduces centriolar satellite density and impedes centrosome amplification.

Europe PMC ID: 28100636


Add a reference

References: 2 entries

Flanagan et al. 2017. Mol Biol Cell. :. PMID: 28100636.

IHC WB

Centriole splitting caused by loss of the centrosomal linker protein C-NAP1 reduces centriolar satellite density and impedes centrosome amplification.

Europe PMC ID: 28100636


Add a reference

References: 2 entries

Flanagan et al. 2017. Mol Biol Cell. :. PMID: 28100636.

IHC WB

Centriole splitting caused by loss of the centrosomal linker protein C-NAP1 reduces centriolar satellite density and impedes centrosome amplification.


Add a reference


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