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13C Metabolite Standard Yeast Extract Small Molecule (Tool Compound)

Invented by Dr Gerrit Hermann at University of Vienna

Info

Synonyms 13C
Relevance Yeast cells are grown on an isotopically enriched 13C growth media prior to harvest when an isotopically enriched extract of the metabolome is obtained and exploited as an internal standard. More than 99% of all carbon atoms have been substituted by stable 13C isotopes.

Can be used as an internal standard for Liquid chromatography–mass spectrometry based amino acid quantification in human plasma.
Research Area Metabolism
Storage -80ºC
Notes Unit size: extract of 2 billion yeast cells

Contents per unit:
Lysine 44 ± 4 nmol
Histidine 90 ± 9 nmol
Arginine 930 ± 40 nmol

References

There are 8 reference entries for this reagent.

View All References

References: 8 entries

Neubauer et al. 2015. Anal Bioanal Chem. 407(22):6681-8. PMID: 26168961.

LC-MS/MS-based analysis of coenzyme A and short-chain acyl-coenzyme A thioesters.

Europe PMC ID: 26168961

Ortmayr et al. 2015. Analyst. 140(10):3465-73. PMID: 25824707.

Complementing reversed-phase selectivity with porous graphitized carbon to increase the metabolome coverage in an on-line two-dimensional LC-MS setup for metabolomics.

Europe PMC ID: 25824707

Klavins et al. 2014. Anal Chem. 86(9):4145-50. PMID: 24678888.

Quantitative metabolite profiling utilizing parallel column analysis for simultaneous reversed-phase and hydrophilic interaction liquid chromatography separations combined with tandem mass spectrometry.

Europe PMC ID: 24678888

Neubauer et al. 2012. J Sep Sci. 35(22):3091-105. PMID: 23086617.

U13C cell extract of Pichia pastoris--a powerful tool for evaluation of sample preparation in metabolomics.

Europe PMC ID: 23086617


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References: 8 entries

Neubauer et al. 2015. Anal Bioanal Chem. 407(22):6681-8. PMID: 26168961.

LC-MS/MS-based analysis of coenzyme A and short-chain acyl-coenzyme A thioesters.

Ortmayr et al. 2015. Analyst. 140(10):3465-73. PMID: 25824707.

Complementing reversed-phase selectivity with porous graphitized carbon to increase the metabolome coverage in an on-line two-dimensional LC-MS setup for metabolomics.

Klavins et al. 2014. Anal Chem. 86(9):4145-50. PMID: 24678888.

Quantitative metabolite profiling utilizing parallel column analysis for simultaneous reversed-phase and hydrophilic interaction liquid chromatography separations combined with tandem mass spectrometry.

Neubauer et al. 2012. J Sep Sci. 35(22):3091-105. PMID: 23086617.

U13C cell extract of Pichia pastoris--a powerful tool for evaluation of sample preparation in metabolomics.


Add a reference