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Anti-5-Hydroxymethylcytosine (5hmc) [AB3/63.3]

Invented by Prof Wolf Reik at Babraham Institute

Info

Catalogue Number 152733
Applications IF IP DB
Antigen/Gene or Protein Targets 5-Hydroxymethylcytosine (5hmC)
Reactivity All
Relevance The anti-5-Hydroxymethylcytosine antibody, clone AB3/63.3, detects 5-hydroxymethyl cytosine (hmc, 5hmc), but not 5-methylcytosine or unmethylated cytosine. 5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base. It is formed from the DNA base cytosine by adding a methyl group and then a hydroxy group. Every mammalian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; generally, the levels of 5-hydroxymethylcytosine increase with age. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethylcytosine may regulate gene expression or initiate DNA demethylation.
Host Rat
Immunogen 5-Hydroxymethylcytosine conjugated to BSA
Subclass IgG2a kappa
Positive Control Unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA
Notes Evaluated by Dot Blot in a panel of unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA.
Dot Blot Analysis: 10 µg/mL and 1 µg/mL of this antibody detected 5-hydroxymethylcytosine in a panel of unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA.

Non-species specific antibody against modified DNA. Expected to detect 5-hydroxymethylcytosine in all species.
Research Area DNA Damage and Repair, Epigenetics & Nuclear Signalling, Gene Expression, Stem Cell Biology

References: 4 entries

Zhang et al. 2014. PLoS One. 9(1):e84925. PMID: 24427299.

IP

Cao et al. 2014. Theriogenology. 81(3):496-508. PMID: 24315686.

Ficz et al. 2011. Nature. 473(7347):398-402. PMID: 21460836.

IF

Wossidlo et al. 2011. Nat Commun. 2:241. PMID: 21407207.


Add a reference

References: 4 entries

Zhang et al. 2014. PLoS One. 9(1):e84925. PMID: 24427299.

IP

Cao et al. 2014. Theriogenology. 81(3):496-508. PMID: 24315686.

Ficz et al. 2011. Nature. 473(7347):398-402. PMID: 21460836.

IF

Wossidlo et al. 2011. Nat Commun. 2:241. PMID: 21407207.


Add a reference

References: 4 entries

Zhang et al. 2014. PLoS One. 9(1):e84925. PMID: 24427299.

IP

Cao et al. 2014. Theriogenology. 81(3):496-508. PMID: 24315686.

Ficz et al. 2011. Nature. 473(7347):398-402. PMID: 21460836.

IF

Wossidlo et al. 2011. Nat Commun. 2:241. PMID: 21407207.


Add a reference


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