Anti-5-Hydroxymethylcytosine (5hmc) [AB3/63.3]
Invented by Prof Wolf Reik from Babraham Institute
Invented at Babraham Institute
- Datasheet
- References (8)
- Inventor Info
Info
![Anti-5-Hydroxymethylcytosine (5hmc) [AB3/63.3] has been used for hydroxymethylated DNA immunoprecipitation (hMeDIP) with enrichment of the target compared to negative controls. (Zhang et al. 2014. PLoS One. 9(1):e84925. PMID: 24427299)](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/a1fe6938-42da-4122-820d-ddf6f5bc8355.jpg)
![Anti-5-Hydroxymethylcytosine (5hmc) [AB3/63.3] has been used for hydroxymethylated DNA immunoprecipitation (hMeDIP) with enrichment of the target compared to negative controls. (Zhang et al. 2014. PLoS One. 9(1):e84925. PMID: 24427299)](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/a1fe6938-42da-4122-820d-ddf6f5bc8355.jpg)
![Image thumbnail for Anti-5-Hydroxymethylcytosine (5hmc) [AB3/63.3]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/a1fe6938-42da-4122-820d-ddf6f5bc8355.jpg)
| Catalogue Number | 152733 |
| Applications | IF IP DB |
| Antigen/Gene or Protein Targets | 5-Hydroxymethylcytosine (5hmC) |
| Reactivity | All |
| Relevance | The anti-5-Hydroxymethylcytosine antibody, clone AB3/63.3, detects 5-hydroxymethyl cytosine (hmc, 5hmc), but not 5-methylcytosine or unmethylated cytosine. 5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base. It is formed from the DNA base cytosine by adding a methyl group and then a hydroxy group. Every mammalian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; generally, the levels of 5-hydroxymethylcytosine increase with age. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethylcytosine may regulate gene expression or initiate DNA demethylation. |
| Host | Rat |
| Immunogen | 5-Hydroxymethylcytosine conjugated to BSA |
| Positive Control | Unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA |
| Subclass | IgG2a kappa |
| Notes |
Evaluated by Dot Blot in a panel of unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA. Dot Blot Analysis: 10 µg/mL and 1 µg/mL of this antibody detected 5-hydroxymethylcytosine in a panel of unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA. Non-species specific antibody against modified DNA. Expected to detect 5-hydroxymethylcytosine in all species. |
| Research Area | DNA Damage and Repair, Epigenetics & Nuclear Signalling, Gene Expression, Stem Cell Biology |
References: 8 entries
Zhang et al. 2014. PLoS One. 9(1):e84925. PMID: 24427299.
IP
Cao et al. 2014. Theriogenology. 81(3):496-508. PMID: 24315686.
Alterations of epigenetic signatures in hepatocyte nuclear factor 4α deficient mouse liver determined by improved ChIP-qPCR and (h)MeDIP-qPCR assays.
Europe PMC ID: 24427299
Dynamic reprogramming of 5-hydroxymethylcytosine during early porcine embryogenesis.
Europe PMC ID: 24315686
Ficz et al. 2011. Nature. 473(7347):398-402. PMID: 21460836.
IF
Dynamic regulation of 5-hydroxymethylcytosine in mouse ES cells and during differentiation.
Europe PMC ID: 21460836
Wossidlo et al. 2011. Nat Commun. 2:241. PMID: 21407207.
5-Hydroxymethylcytosine in the mammalian zygote is linked with epigenetic reprogramming.
Europe PMC ID: 21407207
Add a reference
References: 8 entries
Zhang et al. 2014. PLoS One. 9(1):e84925. PMID: 24427299.
IP
Cao et al. 2014. Theriogenology. 81(3):496-508. PMID: 24315686.
Alterations of epigenetic signatures in hepatocyte nuclear factor 4α deficient mouse liver determined by improved ChIP-qPCR and (h)MeDIP-qPCR assays.
Dynamic reprogramming of 5-hydroxymethylcytosine during early porcine embryogenesis.
Ficz et al. 2011. Nature. 473(7347):398-402. PMID: 21460836.
IF
Dynamic regulation of 5-hydroxymethylcytosine in mouse ES cells and during differentiation.
Wossidlo et al. 2011. Nat Commun. 2:241. PMID: 21407207.
5-Hydroxymethylcytosine in the mammalian zygote is linked with epigenetic reprogramming.
Add a reference
