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Anti-Cisplatin modified DNA, Recombinant [CP9/19]

Invented by Dr Michael Tilby at Absolute Antibody, University of Newcastle upon Tyne, The Institute of Cancer Research

Info

Catalogue Number 152732
Applications ELISA IHC IP DB
Antigen/Gene or Protein Targets Cisplatin modified native DNA
Reactivity N/A
Relevance This antibody enables the quantification of cisplatin-induced adducts on DNA. This antibody has also been recently used for isolation of DNA fragments carrying adducts to enhance the sensitivity of subsequent PCR-based analyses and is central to ongoing studies of variation in the nature of cisplatin adducts formed in different cell lines. Antibody CP9/19 recognises only the intra-strand cross-links formed by cisplatin between adjacent purines.
Host Rat
Subclass IgG2a kappa
Positive Control RNA/DNA
Research Area DNA Damage and Repair, Epigenetics & Nuclear Signalling
Notes Recombinant monoclonal antibody produced from the original monoclonal. Manufactured using Absolute Antibody’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma.

Three different variants available:
- Anti-Cisplatin modified DNA [CP9/19], Rat IgG2a, Kappa
- Anti-Cisplatin modified DNA [CP9/19], Rat IgG2a, Kappa, containing key point mutations that abrogate binding to Fc gamma receptors
- Anti-Cisplatin modified DNA [CP9/19], Rabbit IgG, Kappa.

References

There are 2 reference entries for this reagent.

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References: 2 entries

Sensitive detection of DNA modifications induced by cisplatin and carboplatin in vitro and in vivo using a monoclonal antibody.

Europe PMC ID: 1703029

Original hybridoma first published in: Tilby et al. 1991. Cancer Res. 51(1):123-9. PMID: 1703029.


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References: 2 entries

Sensitive detection of DNA modifications induced by cisplatin and carboplatin in vitro and in vivo using a monoclonal antibody.

Original hybridoma first published in: Tilby et al. 1991. Cancer Res. 51(1):123-9. PMID: 1703029.


Add a reference