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MCF7/S9 Cell Line

Invented by Dr Anne Lykkesfeldt at Danish Cancer Society

Info

Catalogue Number 152602
Parental Line MCF7
Host Human
Tissue Breast
Disease Keywords Breast cancer
Relevance The MCF7/S9 cell line was established to grow in serum free medium to enable studies of the effect of estrogens and antiestrogens under conditions without influence of estrogenic compounds supplied via the serum content in the growth medium. We did not find growth stimulation with estradiol, but after the discovery that phenol red had estrogenic activity, the MCF7/S9 cell line was transferred to phenol red free medium, and in this medium. we observed estrogen stimulation of cell growth.
Production Details MCF7/S9 has been established by gradually adapting MCF7 cells to grow at a low serum concentration before transfer to serum-free medium. Since passage no. 387, the cell line has been propagated in phenol red-free DMEM/F12 (1:1) without addition of serum or any growth factor (standard medium for MCF7/S9), in T-flasks coated with collagen. The MCF7/S9 was subcultured once a week with a split ratio of approximately 1:10 and growth medium was changed every second or third day.
Conditional No
Research Area Cancer, Drug Discovery & Development
Growth/Phenotype Keywords Grow in serum free medium. Estrogen and progesterone receptor positive. Estrogen and antiestrogen responsive.
Recommended Growing Conditions Phenol-red-free DMEM/F12 medium supplemented with 2.0 mM Glutamax, 10 ug/ml Transferrin, 0.1 mg/ml bovine serum albumin, culture flasks were coated with collagen IV.
Cellosaurus ID CVCL_AS09

References: 2 entries

Jensen et al. 2003. J Steroid Biochem Mol Biol. 84(4):469-78. PMID: 12732292.

Briand et al. 1986. Anticancer Res. 6(1):85-90. PMID: 3513694.


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References: 2 entries

Jensen et al. 2003. J Steroid Biochem Mol Biol. 84(4):469-78. PMID: 12732292.

Briand et al. 1986. Anticancer Res. 6(1):85-90. PMID: 3513694.


Add a reference

References: 2 entries

Jensen et al. 2003. J Steroid Biochem Mol Biol. 84(4):469-78. PMID: 12732292.

Briand et al. 1986. Anticancer Res. 6(1):85-90. PMID: 3513694.


Add a reference


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