MCF7/S0.5 Cell Line
Invented by Dr Anne Lykkesfeldt at Danish Cancer Society
MCF7/S9 Cell Line
Invented by Dr Anne Lykkesfeldt from Danish Cancer Society
Invented at Danish Cancer Society
- Datasheet
- References (4)
- Inventor Info
Info
| Catalogue Number | 152602 |
| Parental Line | MCF7 |
| Host | Human |
| Tissue | Breast |
| Disease Keywords | Breast cancer |
| Relevance | The MCF7/S9 cell line was established to grow in serum free medium to enable studies of the effect of estrogens and antiestrogens under conditions without influence of estrogenic compounds supplied via the serum content in the growth medium. We did not find growth stimulation with estradiol, but after the discovery that phenol red had estrogenic activity, the MCF7/S9 cell line was transferred to phenol red free medium, and in this medium. we observed estrogen stimulation of cell growth. |
| Production Details | MCF7/S9 has been established by gradually adapting MCF7 cells to grow at a low serum concentration before transfer to serum-free medium. Since passage no. 387, the cell line has been propagated in phenol red-free DMEM/F12 (1:1) without addition of serum or any growth factor (standard medium for MCF7/S9), in T-flasks coated with collagen. The MCF7/S9 was subcultured once a week with a split ratio of approximately 1:10 and growth medium was changed every second or third day. |
| Conditional | No |
| Research Area | Cancer, Drug Discovery & Development |
| Growth/Phenotype Keywords | Grow in serum free medium. Estrogen and progesterone receptor positive. Estrogen and antiestrogen responsive. |
| Recommended Growing Conditions | Phenol-red-free DMEM/F12 medium supplemented with 2.0 mM Glutamax, 10 ug/ml Transferrin, 0.1 mg/ml bovine serum albumin, culture flasks were coated with collagen IV. |
| Cellosaurus ID | CVCL_AS09 |
References: 4 entries
Jensen et al. 2003. J Steroid Biochem Mol Biol. 84(4):469-78. PMID: 12732292.
Effect of antiestrogens and aromatase inhibitor on basal growth of the human breast cancer cell line MCF-7 in serum-free medium.
Europe PMC ID: 12732292
Briand et al. 1986. Anticancer Res. 6(1):85-90. PMID: 3513694.
Long-term cultivation of a human breast cancer cell line, MCF-7, in a chemically defined medium. Effect of estradiol.
Europe PMC ID: 3513694
Add a reference
References: 4 entries
Jensen et al. 2003. J Steroid Biochem Mol Biol. 84(4):469-78. PMID: 12732292.
Effect of antiestrogens and aromatase inhibitor on basal growth of the human breast cancer cell line MCF-7 in serum-free medium.
Briand et al. 1986. Anticancer Res. 6(1):85-90. PMID: 3513694.
Long-term cultivation of a human breast cancer cell line, MCF-7, in a chemically defined medium. Effect of estradiol.
Add a reference
