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MCF7/ExeR-1 Cell Line

Invented by Dr Anne Lykkesfeldt from Danish Cancer Society
Invented at Danish Cancer Society

Info

Catalogue Number 152555
Antigen/Gene or Protein Targets Exemestane resistance
Parental Line MCF7
Host Human
Tissue Breast
Disease Keywords Breast Cancer
Relevance The MCF7/ExeR-1 Cell Line was developed as a model of resistance to anti-cancer treatment with aromatase inhibitors.Third generation aromatase inhibitors (AIs) have proven to be effective treatment for estrogen receptor positive (ER+) breast cancer and are today recommended as first line endocrine therapy for postmenopausal ER+ breast cancer patients, making up the majority of breast cancer patients. However, a major problem is development of resistance against AIs. Since molecular mechanisms of AI resistance are largely undisclosed, the development of cell lines resistant to the steroidal AI exemestane allows the study of the molecular basis for resistance to AIs to unravel new targets for treatment.
Production Details Exemestane-resistant cell lines were established from MCF-7 cells grown in medium with 10% NCS and 10−7 M testosterone. A culture of MCF-7 cells were treated with 10−7 M exemestane for one week, trypsinized and seeded in serial dilutions in 24-well plates. Single colonies were transferred to new wells and gradually expanded in medium with exemestane. After ~2–3 months, the isolated colonies gave rise to exemestane-resistant cell lines, which could be grown in exemestane-containing medium with a weekly split ratio of ~1:25. The MCF-7 cell line was authenticated in January 2014 by DNA profiling using short tandem repeat loci and found to be matching the genetic profile reported for the MCF-7 cell line.
Conditional No
Research Area Cancer, Drug Discovery & Development
Growth/Phenotype Keywords Breast cancer cell line resistant to the aromatase inhibitor exemestane. Estrogen receptor positive. Progesterone receptor positive when grown in medium without exemestane.
Recommended Growing Conditions Phenol-red-free DMEM/F12 medium supplemented with 10% newborn calf serum, 2.5 mM Glutamax, 6 ng/ ml insulin, 0.1 uM testosterone and 0.1 uM exemestane.
Notes Human breast cancer cell line derived from MCF-7 cells
Other related cell lines:
- LetR-1, LetR-2, LetR-3 and LetR-4 resistant to the non-steroidal AI letrozole
- ExeR-2, ExeR-3 and ExeR-4 resistant to the steroidal AI exemestane
- AnaR-1, AnaR-2, AnaR-3 and AnaR-4 resistant to the non-steroidal AI anastrozole

Passage 430 (AL3058, AL3059)
Cellosaurus ID CVCL_5A13

References

There are 4 reference entries for this reagent.

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References: 4 entries

Hole et al. 2015. Breast Cancer Res Treat. 149(3):715-26. PMID: 25667100.

Hole et al. 2015. Int J Oncol. 46(4):1481-90. PMID: 25625755.

Aurora kinase A and B as new treatment targets in aromatase inhibitor-resistant breast cancer cells.

Europe PMC ID: 25667100

New cell culture model for aromatase inhibitor-resistant breast cancer shows sensitivity to fulvestrant treatment and cross-resistance between letrozole and exemestane.

Europe PMC ID: 25625755


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References: 4 entries

Hole et al. 2015. Breast Cancer Res Treat. 149(3):715-26. PMID: 25667100.

Hole et al. 2015. Int J Oncol. 46(4):1481-90. PMID: 25625755.

Aurora kinase A and B as new treatment targets in aromatase inhibitor-resistant breast cancer cells.

New cell culture model for aromatase inhibitor-resistant breast cancer shows sensitivity to fulvestrant treatment and cross-resistance between letrozole and exemestane.


Add a reference