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MCF7/LetR-4 Cell Line

Invented by Dr Anne Lykkesfeldt at Danish Cancer Society

Info

Catalogue Number 152550
Antigen/Gene or Protein Targets Letrozole resistant
Parental Line MCF7
Host Human
Tissue Breast
Disease Keywords Breast Cancer
Relevance The MCF7/LetR-4 Cell Line was developed as a model of resistance to anti-cancer treatment with aromatase inhibitors. Third generation aromatase inhibitors (AIs) have proven to be effective treatment for estrogen receptor positive (ER+) breast cancer and are today recommended as first line endocrine therapy for postmenopausal ER+ breast cancer patients, making up the majority of breast cancer patients. However, a major problem is development of resistance against AIs. Since molecular mechanisms of AI resistance are largely undisclosed, the development of cell lines resistant to the non-steroidal AI letrozole allows the study of the molecular basis for resistance to AIs to unravel new targets for treatment.
Production Details Letrozole-resistant cell lines were established from MCF-7 cells grown in medium with 10% NCS and 10−7 M testosterone. A culture of MCF-7 cells were treated with 10−6 M letrozole for one week, trypsinized and seeded in serial dilutions in 24-well plates. Single colonies were transferred to new wells and gradually expanded in medium with letrozole. After ~2–3 months, the isolated colonies gave rise to letrozole-resistant cell lines, which could be grown in letrozole-containing medium with a weekly split ratio of ~1:25. The MCF-7 cell line was authenticated in January 2014 by DNA profiling using short tandem repeat loci and found to be matching the genetic profile reported for the MCF-7 cell line.
Conditional No
Research Area Cancer, Drug Discovery & Development
Growth/Phenotype Keywords Breast cancer cell line resistant to the aromatase inhibitor letrozole. Estrogen receptor positive.
Recommended Growing Conditions Phenol-red-free DMEM/F12 medium supplemented with 10% newborn calf serum, 2.5 mM Glutamax, 6 ng/ ml insulin, 0.1 uM testosterone and 1 uM letrozole.
Notes Human breast cancer cell line derived from MCF-7 cells
Other related cell lines:
- LetR-1, LetR-2 and LetR-3 resistant to the non-steroidal AI letrozole
- ExeR-1, ExeR-2, ExeR-3 and ExeR-4 resistant to the steroidal AI exemestane
- AnaR-1, AnaR-2, AnaR-3 and AnaR-4 resistant to the non-steroidal AI anastrozole

Passage 436 (AL3183, AL3184)
Cellosaurus ID CVCL_5A20

References: 2 entries

Hole et al. 2015. Breast Cancer Res Treat. 149(3):715-26. PMID: 25667100.

Hole et al. 2015. Int J Oncol. 46(4):1481-90. PMID: 25625755.


Add a reference

References: 2 entries

Hole et al. 2015. Breast Cancer Res Treat. 149(3):715-26. PMID: 25667100.

Hole et al. 2015. Int J Oncol. 46(4):1481-90. PMID: 25625755.


Add a reference

References: 2 entries

Hole et al. 2015. Breast Cancer Res Treat. 149(3):715-26. PMID: 25667100.

Hole et al. 2015. Int J Oncol. 46(4):1481-90. PMID: 25625755.


Add a reference


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