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pcDNA5/FRT/TO(Neo)-Venus-Flag-WT wobbled cdc20 (1991) Vector

Invented by Dr Jonathon Pines at Gurdon Institute

Info

Antigen/Gene or Protein Targets cdc20
Relevance Gene/Insert name cdc20
Species H. sapiens (human)
Mutation wobbled
Promoter CMV
Tags / Fusion Proteins
Flag (N terminal on backbone)
Venus (N terminal on backbone)

CLONING INFORMATION
Cloning method Unknown
5′ sequencing primer CMV-F
3′ sequencing primer BGHrev
Research Area Protein Degradation
Notes Vector backbone pcDNA5/FRT/TO(Neo)-Venus-Flag
Vector type Mammalian Expression
Selectable markers Neomycin

GROWTH IN BACTERIA
Bacterial Resistance(s) Ampicillin
Growth Temperature 37°C
Growth Strain(s) DH5alpha
Copy number High Copy

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