Endoglin Floxed Mouse
Invented at University of Newcastle upon Tyne
- Datasheet
- References (6)
- Inventor Info
Info
| Catalogue Number | 151754 |
| Antigen/Gene or Protein Targets | Endoglin |
| Disease Keywords | Hereditary Haemorrhagic Telangiectasia; angiogenesis defects; fibrosis defects |
| Relevance | Endoglin is an auxiliary receptor for TGFb signalling. Heterozygous germline Endoglin mutations have been identified in patients with the vascular abnormality, Hereditary Haemorrhagic Telangiectasia. Endoglin is upregulated in endothelial cells during angiogenesis and the loss of Endoglin in [transgenic] mice results in embryonic lethality at mid-gestation. This phenotype points to an important role of Endoglin in new blood vessel formation but precludes analysis at later stages in development and in postnatal life. To bypass this limitation and allow further investigations of the function of Endoglin a transgenic mice has been generated with a floxed Endoglin allele in which loxP sites flank exons 5 and 6. Mice homozygous for this allele are normal and in the presence of appropriate Cre lines will allow time and cell specific Endoglin deletion for in vivo analysis of function in cardiovascular development and disease. |
| Production Details | Targeting the endoglin gene with trifloxed (3fl) eng vector by homologous recombination in mouse ES cells. Endoglin exons 5 and 6 and a PGK-neo selection cassette are flanked by loxP sites. The neo cassette was removed from heterozygous Eng3fl/รพ mice in vivo using the Meu-Cre40 transgenic mouse. These mosaics were then crossed with wild type C57Bl/6 females to generate F2 mice and all possible recombination events were observed in the F2 generation. F2 mice had inherited the floxed Endoglin allele, but not the Meu-Cre40 transgene. |
| Conditional | Yes |
| Conditional Description | Floxed allele |
| Growth/Phenotype Keywords | No phenotype in the absence of Cre activity |
| Mouse Genetic Background/Cross History | C57Bl/6 (4 generations of backcrossing) |
| Research Area | Cancer, Cardiovascular, Cell Type or Organelle Marker, Genetic Studies Tools, Immunology, Stem Cell Biology |
References: 6 entries
Tual-Chalot et al. 2020. Circ Res. 126(2):243-257. PMID: 31805812.
Anderberg et al. 2013. J Exp Med. 210(3):563-79. PMID: 23401487.
Deficiency for endoglin in tumor vasculature weakens the endothelial barrier to metastatic dissemination.
Europe PMC ID: 23401487
Mahmoud et al. 2010. Circ Res. 106(8):1425-33. PMID: 20224041.
Allinson et al. 2007. Genesis. 45(6):391-5. PMID: 17506087.
Generation of a floxed allele of the mouse Endoglin gene.
Europe PMC ID: 17506087
Add a reference
References: 6 entries
Tual-Chalot et al. 2020. Circ Res. 126(2):243-257. PMID: 31805812.
Anderberg et al. 2013. J Exp Med. 210(3):563-79. PMID: 23401487.
Deficiency for endoglin in tumor vasculature weakens the endothelial barrier to metastatic dissemination.
Mahmoud et al. 2010. Circ Res. 106(8):1425-33. PMID: 20224041.
Allinson et al. 2007. Genesis. 45(6):391-5. PMID: 17506087.
Generation of a floxed allele of the mouse Endoglin gene.
Add a reference
