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HEK293 GFP-WIPI2 Cell Line

Invented by Dr Sharon Tooze at The Francis Crick Institute

Info

Catalogue Number 151716
Antigen/Gene or Protein Targets Human WIPI2, GFP
Parental Line HEK293
Host Human
Tissue Embryo
Disease Keywords Cancer, neurodegeneration
Model Transgenic
Relevance The HEK293 GFP-WIPI2 cell line can be used to study autophagy by light microscopy.It is also useful for high throughput microscopy screens. Stable cell line expressing GFP-tagged human WIPI2 in background of HEK293A cell line
Production Details HEK293 cells were transfected with GFP-WIPI2 then selcted with G418, and stable, amino acid responsive clones were selected.
Research Area Apoptosis and Programmed Cell Death, Cancer, Cell Signaling & Signal Transduction, Cellular Transport, Drug Discovery & Development, Metabolism
Growth/Phenotype Keywords Autophagy
Recommended Growing Conditions DMEM with 10% FCS
Notes HEK293 cells were obtained under licence from AdVec
Cellosaurus ID CVCL_5A21

References: 1 entry

Dooley et al. 2014. Mol Cell. 55(2):238-52. PMID: 24954904.


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References: 1 entry

Dooley et al. 2014. Mol Cell. 55(2):238-52. PMID: 24954904.


Add a reference

References: 1 entry

Dooley et al. 2014. Mol Cell. 55(2):238-52. PMID: 24954904.


Add a reference


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