HEK293-A GFP-WIPI2 Cell Line
Invented by Dr Sharon Tooze from The Francis Crick Institute
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 151716 |
| Antigen/Gene or Protein Targets | Human WIPI2, GFP |
| Parental Line | HEK293-A |
| Host | Human |
| Tissue | Embryo |
| Disease Keywords | Cancer, neurodegeneration |
| Model | Transgenic |
| Relevance | The HEK293-A GFP-WIPI2 cell line can be used to study autophagy by light microscopy.It is also useful for high throughput microscopy screens. Stable cell line expressing GFP-tagged human WIPI2 in background of HEK293-A cell line |
| Production Details | HEK293-A cells were transfected with GFP-WIPI2 then selcted with G418, and stable, amino acid responsive clones were selected. |
| Research Area | Apoptosis and Programmed Cell Death, Cancer, Cell Signaling & Signal Transduction, Cellular Transport, Drug Discovery & Development, Metabolism |
| Growth/Phenotype Keywords | Autophagy |
| Recommended Growing Conditions | DMEM with 10% FCS |
| Notes | HEK293 cells were obtained under licence from AdVec |
| Cellosaurus ID | CVCL_5A21 |
References: 2 entries
Dooley et al. 2014. Mol Cell. 55(2):238-52. PMID: 24954904.
WIPI2 links LC3 conjugation with PI3P, autophagosome formation, and pathogen clearance by recruiting Atg12-5-16L1.
Europe PMC ID: 24954904
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References: 2 entries
Dooley et al. 2014. Mol Cell. 55(2):238-52. PMID: 24954904.
WIPI2 links LC3 conjugation with PI3P, autophagosome formation, and pathogen clearance by recruiting Atg12-5-16L1.
Add a reference
