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Anti-von Willebrand Factor [RFF-VIII:R/2]

Info

Catalogue Number 151527
Applications ELISA FACS IHC IF IP Fn RIA WB
Antigen/Gene or Protein Targets Von Willebrand factor (VWF)
Reactivity Primate
Relevance The monoclonal antibody RFF-VIII:R/1 specifically binds to human factor VIII related antigen (VIII:RAg) in plasma and in vascular endothelial cells but has no reactivity with factor VIII procoagulant antigen (VIII:cAg). This antibody is a potent inhibitor of von Willebrand factor activity in that it can totally neutralise risocetin-induced aggregation of platelet rich plasma and inhibit platelet adhesion at high flow rates. RFF-VIII:R/1 can be used in a one-stage, fluid phase immunoradiometric assay that can detect VIII:RAg.

This monoclonal antibody binds to human von Willebrand factor (VW) and inhibits it's binding to platelets. Very similar in characteristics to RFF-VIII:R/2
Host Mouse
Immunogen Purified human Factor VIII-VWF complex
Subclass IgG1 kappa
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI + 10% FCS; non-adherent; subculture every 2-3 days; split 1:5
Notes ELISA 1:100-1:2000
Immunohistochemistry 1:10-1:2000
Immunohistochemistry-Frozen 1:25-1:100
Protein Array 1:100-1:2000
Radioimmunoassay 1:100-1:2000
Research Area Cardiovascular, Stem Cell Biology

References: 7 entries

[Use of RFF-VIII:R/1 in the purification of FVIII]

Murdock et al. 1997. Thromb Haemost. 78(4):1272-7. PMID: 9364997.

Takase et al. 1987. Br J Haematol. 66(4):497-502. PMID: 3117089.

McCraw et al. 1987. Thromb Res. 45(1):101-8. PMID: 3494325.

Chand et al. 1986. Thromb Haemost. 55(3):318-24. PMID: 2428124.

Rotblat et al. 1985. Biochemistry. 24(16):4294-300. PMID: 2413885.

Goodall et al. 1985. Br J Haematol. 59(4):565-77. PMID: 2580547.


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