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Anti-Melphalan-modified DNA [MP5/73]

Invented by Dr Michael Tilby at University of Newcastle upon Tyne

Info

Catalogue Number 151466
Applications ELISA IHC
Antigen/Gene or Protein Targets Melphalan
Reactivity N/A
Relevance This antibody was developed to improve detection of melphalan-DNA adducts through its ability to recognise the stabilised adduct. This antibody can be used for quantification of melphalan adducts in purified DNA by ELISA and for immunofluorescent detection of melphalan-DNA adducts in single cells.
Host Rat
Immunogen Native DNA reacted with melphalan
Subclass IgG2b
Positive Control RNA/DNA
Myeloma Used Y3.AG.1.2.3
Recommended Growing Conditions DMEM + 10%FCS + Glu + Pen/Strep
Research Area Cancer

References: 18 entries

Gould et al. 2004. Mol Pharmacol. 66(5):1301-9. PMID: 15308759.

p53 elevation in relation to levels and cytotoxicity of mono- and bifunctional melphalan-DNA adducts.

Europe PMC ID: 15308759

Rothbarth et al. 2004. Biochem Pharmacol. 67(9):1771-8. PMID: 15081876.

Immunohistochemical detection of melphalan-DNA adducts in colon cancer cells in vitro and human colorectal liver tumours in vivo.

Europe PMC ID: 15081876

Frank et al. 2003. Exp Cell Res. 283(2):127-34. PMID: 12581733.

Quantification of DNA adducts in individual cells by immunofluorescence: effects of variation in DNA conformation.

Europe PMC ID: 12581733

McCartney et al. 2001. Chem Res Toxicol. 14(1):71-81. PMID: 11170510.

Antibody recognition of melphalan adducts characterized using immobilized DNA: enhanced alkylation of G-Rich regions in cells compared to in vitro.

Europe PMC ID: 11170510

Tilby et al. 1998. Chem Res Toxicol. 11(10):1162-8. PMID: 9778312.

A monofunctional derivative of melphalan: preparation, DNA alkylation products, and determination of the specificity of monoclonal antibodies that recognize melphalan-DNA adducts.

Europe PMC ID: 9778312

Frank et al. 1996. Blood. 88(3):977-84. PMID: 8704257.

Detection and quantification of melphalan-DNA adducts at the single cell level in hematopoietic tumor cells.

Europe PMC ID: 8704257

Tilby et al. 1993. Eur J Cancer. 29A(5):681-6. PMID: 8471325.

Application of a sensitive immunoassay to the study of DNA adducts formed in peripheral blood mononuclear cells of patients undergoing high-dose melphalan therapy.

Europe PMC ID: 8471325

Tilby et al. 1990. Chem Biol Interact. 73(2-3):183-94. PMID: 2155711.

Alkylation of DNA by melphalan in relation to immunoassay of melphalan-DNA adducts: characterization of mono-alkylated and cross-linked products from reaction of melphalan with dGMP and GMP.

Europe PMC ID: 2155711

Tilby et al. 1987. Cancer Res. 47(6):1542-6. PMID: 3815354.

Immunological detection of DNA damage caused by melphalan using monoclonal antibodies.

Europe PMC ID: 3815354


Add a reference

References: 18 entries

Gould et al. 2004. Mol Pharmacol. 66(5):1301-9. PMID: 15308759.

p53 elevation in relation to levels and cytotoxicity of mono- and bifunctional melphalan-DNA adducts.

Europe PMC ID: 15308759

Rothbarth et al. 2004. Biochem Pharmacol. 67(9):1771-8. PMID: 15081876.

Immunohistochemical detection of melphalan-DNA adducts in colon cancer cells in vitro and human colorectal liver tumours in vivo.

Europe PMC ID: 15081876

Frank et al. 2003. Exp Cell Res. 283(2):127-34. PMID: 12581733.

Quantification of DNA adducts in individual cells by immunofluorescence: effects of variation in DNA conformation.

Europe PMC ID: 12581733

McCartney et al. 2001. Chem Res Toxicol. 14(1):71-81. PMID: 11170510.

Antibody recognition of melphalan adducts characterized using immobilized DNA: enhanced alkylation of G-Rich regions in cells compared to in vitro.

Europe PMC ID: 11170510

Tilby et al. 1998. Chem Res Toxicol. 11(10):1162-8. PMID: 9778312.

A monofunctional derivative of melphalan: preparation, DNA alkylation products, and determination of the specificity of monoclonal antibodies that recognize melphalan-DNA adducts.

Europe PMC ID: 9778312

Frank et al. 1996. Blood. 88(3):977-84. PMID: 8704257.

Detection and quantification of melphalan-DNA adducts at the single cell level in hematopoietic tumor cells.

Europe PMC ID: 8704257

Tilby et al. 1993. Eur J Cancer. 29A(5):681-6. PMID: 8471325.

Application of a sensitive immunoassay to the study of DNA adducts formed in peripheral blood mononuclear cells of patients undergoing high-dose melphalan therapy.

Europe PMC ID: 8471325

Tilby et al. 1990. Chem Biol Interact. 73(2-3):183-94. PMID: 2155711.

Alkylation of DNA by melphalan in relation to immunoassay of melphalan-DNA adducts: characterization of mono-alkylated and cross-linked products from reaction of melphalan with dGMP and GMP.

Europe PMC ID: 2155711

Tilby et al. 1987. Cancer Res. 47(6):1542-6. PMID: 3815354.

Immunological detection of DNA damage caused by melphalan using monoclonal antibodies.

Europe PMC ID: 3815354


Add a reference

References: 18 entries

Gould et al. 2004. Mol Pharmacol. 66(5):1301-9. PMID: 15308759.

p53 elevation in relation to levels and cytotoxicity of mono- and bifunctional melphalan-DNA adducts.

Rothbarth et al. 2004. Biochem Pharmacol. 67(9):1771-8. PMID: 15081876.

Immunohistochemical detection of melphalan-DNA adducts in colon cancer cells in vitro and human colorectal liver tumours in vivo.

Frank et al. 2003. Exp Cell Res. 283(2):127-34. PMID: 12581733.

Quantification of DNA adducts in individual cells by immunofluorescence: effects of variation in DNA conformation.

McCartney et al. 2001. Chem Res Toxicol. 14(1):71-81. PMID: 11170510.

Antibody recognition of melphalan adducts characterized using immobilized DNA: enhanced alkylation of G-Rich regions in cells compared to in vitro.

Tilby et al. 1998. Chem Res Toxicol. 11(10):1162-8. PMID: 9778312.

A monofunctional derivative of melphalan: preparation, DNA alkylation products, and determination of the specificity of monoclonal antibodies that recognize melphalan-DNA adducts.

Frank et al. 1996. Blood. 88(3):977-84. PMID: 8704257.

Detection and quantification of melphalan-DNA adducts at the single cell level in hematopoietic tumor cells.

Tilby et al. 1993. Eur J Cancer. 29A(5):681-6. PMID: 8471325.

Application of a sensitive immunoassay to the study of DNA adducts formed in peripheral blood mononuclear cells of patients undergoing high-dose melphalan therapy.

Tilby et al. 1990. Chem Biol Interact. 73(2-3):183-94. PMID: 2155711.

Alkylation of DNA by melphalan in relation to immunoassay of melphalan-DNA adducts: characterization of mono-alkylated and cross-linked products from reaction of melphalan with dGMP and GMP.

Tilby et al. 1987. Cancer Res. 47(6):1542-6. PMID: 3815354.

Immunological detection of DNA damage caused by melphalan using monoclonal antibodies.


Add a reference


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