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Anti-PCNA [5E6/2]


Catalogue Number 151456
Applications ELISA IP WB
Antigen/Gene or Protein Targets Proliferating cell nuclear antigen, (PCNA), also known as cyclin or polymerase delta accessory protein.
Reactivity Saccharomyces cerevisiae
Relevance The antibody recognises endogenous PCNA protein from budding yeast, Sacchoromyces cerevisiae. PCNA is the replicative clamp protein, an essential processivity factor for DNA polymerases and an interaction partner for many other proteins. By means of its interactions, PCNA coordinates various transactions at the replication fork, ranging from DNA replication and repair to chromatin assembly.
Host Mouse
Immunogen Purified recombinant His6-POL30 (PCNA) from budding yest, Saccharomyces cerevisiae, produced in Escherichia coli
Subclass IgG1
Positive Control Wild type budding yeast expressing native POL30 (PCNA)
Molecular Weight (kDa) 30
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions RPMI + 5% FCS
Strain CD1
Research Area Cancer, Cell Cycle, Cell Type or Organelle Marker, Epigenetics & Nuclear Signalling

References: 4 entries

Kubota et al. 2011. Mol Cell Proteomics. 10(7):M110.005561. PMID: 21505101.

Parker et al. 2008. EMBO J. 27(18):2422-31. PMID: 18701921.

Davies et al. 2008. Mol Cell. 29(5):625-36. PMID: 18342608.

Windecker et al. 2008. J Mol Biol. 376(1):221-31. PMID: 18155241.

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