Anti-HSP27 [ACD5]
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (8)
- Inventor Info
Info
| Catalogue Number | 151427 |
| Applications | IHC IP |
| Antigen/Gene or Protein Targets | HSP27 |
| Synonyms | Heat Shock Protein Family B (Small) Member 1; Estrogen-Regulated 24 KDa Protein; Stress-Responsive Protein 27; Heat Shock 27kDa Protein; HSP27; HSP28; SRP27; Epididymis Secretory Protein Li 102; HEL-S-102; HS.76067; CMT2F; HMN2B; Hsp25; HspB1 |
| Reactivity | Human |
| Relevance | The anti estrogen receptor (RE) antibody ACD5 (also known as D5) is capable of precipitating labelled human cytosolic RE complexes from breast tumour, fibroid, myometrial and endometrial preparations, but does not react with nuclear RE from human endometrium or cytoplasmic RE from other species tested. The antibody does not react with progesterone receptor or androgen receptor from human breast tissue, sex steroid binding globulin (SHBG) from human plasma, or rat alpha fetoprotein. The antibody is associated with a 29KDa component in RE-positive cytosols, in addition the antigen is a non hormone binding component related to cytosolic RE from breast tumour and myometrium. |
| Host | Mouse |
| Immunogen | Purified cytoplasmic oestrogen receptor fragment from human myometrium. |
| Subclass | IgG1 |
| Molecular Weight (kDa) | 27 |
| Myeloma Used | P3X63Ag8.653 |
| Recommended Growing Conditions | RPMI + 20% FCS, penicillin 100 (units/ml), streptomycin (100ug/ml) |
| Strain | Balb/c |
| Notes | Since publication of the Coffer et al 1985 paper, work has been performed to elucidate the nature of the 29KDa protein. It is an abundant protein that had previously been discussed under three different names, p29, p24 and srp27, but it is known that they are one and the same protein and now called Heat Shock Protein 27, HSP27. |
| Research Area | Cancer, Cardiovascular, Cell Signaling & Signal Transduction |
References: 8 entries
Zhu et al. 1994. J Biol Chem. 269(35):22379-84. PMID: 7915282.
Specific binding of the transglutaminase, platelet factor XIII, to HSP27.
Europe PMC ID: 7915282
Love et al. 1994. Br J Cancer. 69(4):743-8. PMID: 8142264.
A 27 kDa heat shock protein that has anomalous prognostic powers in early and advanced breast cancer.
Europe PMC ID: 8142264
Dunn et al. 1993. J Steroid Biochem Mol Biol. 46(4):469-79. PMID: 8217877.
Relationship of HSP27 and oestrogen receptor in hormone sensitive and insensitive cell lines.
Europe PMC ID: 8217877
Coffer et al. 1985. Cancer Res. 45(8):3686-93. PMID: 4016746.
Monoclonal antibodies against a component related to soluble estrogen receptor.
Europe PMC ID: 4016746
Add a reference
References: 8 entries
Zhu et al. 1994. J Biol Chem. 269(35):22379-84. PMID: 7915282.
Specific binding of the transglutaminase, platelet factor XIII, to HSP27.
Love et al. 1994. Br J Cancer. 69(4):743-8. PMID: 8142264.
A 27 kDa heat shock protein that has anomalous prognostic powers in early and advanced breast cancer.
Dunn et al. 1993. J Steroid Biochem Mol Biol. 46(4):469-79. PMID: 8217877.
Relationship of HSP27 and oestrogen receptor in hormone sensitive and insensitive cell lines.
Coffer et al. 1985. Cancer Res. 45(8):3686-93. PMID: 4016746.
Monoclonal antibodies against a component related to soluble estrogen receptor.
Add a reference
