Anti-BCL2 [bcl-2/100]
Invented by Dr Jacqueline Cordell from University of Oxford
Invented at University of Oxford
- Datasheet
- References (29)
- Inventor Info
Info
| Catalogue Number | 151406 |
| Applications | IHC WB |
| Antigen/Gene or Protein Targets | Bcl2 |
| Reactivity | Human |
| Relevance |
Monoclonal antibody directed against both isoforms of BCL-2 protein. Background and Research Application The pro-survival protein BCL2 derives its name from B-cell lymphoma 2, as it is the second member of a range of proteins initially described in chromosomal translocations involving chromosomes 14 and 18 in follicular lymphomas. There are two isoforms, alpha and beta, of Bcl-2 have been identified and are a result of alternative splicing at the C-terminus. BCL-2 protein is involved in apoptosis, and inhibits the function of pro-apoptotic proteins such as Bax and Bak. Damage to the Bcl-2 gene has been identified as a cause of a number of cancers, including melanoma, breast, prostate, chronic lymphocytic leukaemia, and lung cancer, and a possible cause of schizophrenia and autoimmunity. It is also a cause of resistance to cancer treatments. This antibody recognises both the alpha and beta isoforms of BCL-2. |
| Host | Mouse |
| Immunogen | Synthetic peptide of amino acids 41-54 of human bcl-2 oncoprotein |
| Immunogen UniProt ID | P10415 |
| Subclass | IgG1 |
| Myeloma Used | P3/NS1/1-Ag4.1 |
| Recommended Growing Conditions | RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Concentration |
| Research Area | Apoptosis and Programmed Cell Death, Cancer, Cell Signaling & Signal Transduction, Metabolism |
References: 29 entries
Hirpara et al. 2020. Redox Biol. 30:101403. PMID: 31954371.
Steinbach et al. 2020. PLoS One. 15(1):e0227676. PMID: 31935257.
Jeffery et al. 2017. Clin Exp Immunol. 188(3):394-411. PMID: 28176332.
Soilu-Hänninen et al. 1999. J Neurosci. 19(12):4828-38. PMID: 10366617.
Nerve growth factor signaling through p75 induces apoptosis in Schwann cells via a Bcl-2-independent pathway.
Europe PMC ID: 10366617
Navratil et al. 1995. J Clin Pathol. 48(1):18-21. PMID: 7706514.
Ashton-Key et al. 1995. Histopathology. 26(1):75-8. PMID: 7713486.
Heterogeneity of bcl-2 expression in MALT lymphoma.
Europe PMC ID: 7713486
Expression of the bcl-2 protein in B cell lymphomas arising from mucosa associated lymphoid tissue.
Europe PMC ID: 7706514
Piris et al. 1994. Br J Cancer. 69(2):337-41. PMID: 8297731.
p53 and bcl-2 expression in high-grade B-cell lymphomas: correlation with survival time.
Europe PMC ID: 8297731
Leek et al. 1994. Br J Cancer. 69(1):135-9. PMID: 8286195.
de Jong et al. 1994. Cancer Res. 54(1):256-60. PMID: 8261449.
Subcellular localization of the bcl-2 protein in malignant and normal lymphoid cells.
Europe PMC ID: 8261449
bcl-2 in normal human breast and carcinoma, association with oestrogen receptor-positive, epidermal growth factor receptor-negative tumours and in situ cancer.
Europe PMC ID: 8286195
Pezzella et al. 1993. N Engl J Med. 329(10):690-4. PMID: 8393963.
bcl-2 protein in non-small-cell lung carcinoma.
Europe PMC ID: 8393963
Doussis et al. 1993. Am J Clin Pathol. 99(6):663-7. PMID: 8322700.
An immunocytochemical study of p53 and bcl-2 protein expression in Hodgkin's disease.
Europe PMC ID: 8322700
Pezzella et al. 1993. Histopathology. 22(1):39-44. PMID: 8436340.
Immunohistochemical detection of p53 and bcl-2 proteins in non-Hodgkin's lymphoma.
Europe PMC ID: 8436340
Gaulard et al. 1992. Am J Pathol. 140(5):1089-95. PMID: 1374590.
Expression of the bcl-2 gene product in follicular lymphoma.
Europe PMC ID: 1374590
Pezzella et al. 1992. Br J Cancer. 65(1):87-9. PMID: 1733447.
Evaluation of bcl-2 protein expression and 14;18 translocation as prognostic markers in follicular lymphoma.
Europe PMC ID: 1733447
Pezzella et al. 1990. Lancet. 336(8729):1510-1. PMID: 1979117.
Bcl-2 protein expression in follicular lymphomas in absence of 14;18 translocation.
Europe PMC ID: 1979117
Lehmkuhl et al. 1977. Am J Vet Res. 38(11):1717-20. PMID: 201196.
Investigation of causative agents of bovine respiratory tract disease in a beef cow-calf herd with an early weaning program.
Europe PMC ID: 201196
Add a reference
References: 29 entries
Hirpara et al. 2020. Redox Biol. 30:101403. PMID: 31954371.
Steinbach et al. 2020. PLoS One. 15(1):e0227676. PMID: 31935257.
Jeffery et al. 2017. Clin Exp Immunol. 188(3):394-411. PMID: 28176332.
Soilu-Hänninen et al. 1999. J Neurosci. 19(12):4828-38. PMID: 10366617.
Nerve growth factor signaling through p75 induces apoptosis in Schwann cells via a Bcl-2-independent pathway.
Navratil et al. 1995. J Clin Pathol. 48(1):18-21. PMID: 7706514.
Ashton-Key et al. 1995. Histopathology. 26(1):75-8. PMID: 7713486.
Heterogeneity of bcl-2 expression in MALT lymphoma.
Expression of the bcl-2 protein in B cell lymphomas arising from mucosa associated lymphoid tissue.
Piris et al. 1994. Br J Cancer. 69(2):337-41. PMID: 8297731.
p53 and bcl-2 expression in high-grade B-cell lymphomas: correlation with survival time.
Leek et al. 1994. Br J Cancer. 69(1):135-9. PMID: 8286195.
de Jong et al. 1994. Cancer Res. 54(1):256-60. PMID: 8261449.
Subcellular localization of the bcl-2 protein in malignant and normal lymphoid cells.
bcl-2 in normal human breast and carcinoma, association with oestrogen receptor-positive, epidermal growth factor receptor-negative tumours and in situ cancer.
Pezzella et al. 1993. N Engl J Med. 329(10):690-4. PMID: 8393963.
bcl-2 protein in non-small-cell lung carcinoma.
Doussis et al. 1993. Am J Clin Pathol. 99(6):663-7. PMID: 8322700.
An immunocytochemical study of p53 and bcl-2 protein expression in Hodgkin's disease.
Pezzella et al. 1993. Histopathology. 22(1):39-44. PMID: 8436340.
Immunohistochemical detection of p53 and bcl-2 proteins in non-Hodgkin's lymphoma.
Gaulard et al. 1992. Am J Pathol. 140(5):1089-95. PMID: 1374590.
Expression of the bcl-2 gene product in follicular lymphoma.
Pezzella et al. 1992. Br J Cancer. 65(1):87-9. PMID: 1733447.
Evaluation of bcl-2 protein expression and 14;18 translocation as prognostic markers in follicular lymphoma.
Pezzella et al. 1990. Lancet. 336(8729):1510-1. PMID: 1979117.
Bcl-2 protein expression in follicular lymphomas in absence of 14;18 translocation.
Lehmkuhl et al. 1977. Am J Vet Res. 38(11):1717-20. PMID: 201196.
Investigation of causative agents of bovine respiratory tract disease in a beef cow-calf herd with an early weaning program.
Add a reference
