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Anti-HLADR [CR3/43]

Invented by Dr Jacqueline Cordell at University of Oxford

Info

Catalogue Number 151383
Applications FACS IHC WB
Antigen/Gene or Protein Targets Human Leukocyte Antigen DR (MHC class II)
Reactivity Human
Relevance Monoclonal antibody capable of HLA typing, reacting with subregions of class II antigens.

Background and Research Application
Human Leukocyte Antigens (HLA) are highly polymorphic proteins that are involved in the presentation of antigens to the T-cell receptor. There are two classes of HLA antigens, class I (HLA-A, HLA-B and HLA-C) and class II (HLA-D). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. The human MHC class II molecules bind intracellularly processed peptides and present them to T helper cells. They therefore have a critical role in the initiation of the immune response.
CR3/43 may be used for HLA typing. This antibody reacts with the beta-chain of all products of the gene subregions DP, DQ, and DR.
Host Mouse
Immunogen Tonsil cells
Immunogen UniProt ID P01903
Subclass IgG1
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
HLA-DR was detectable in all of the diffuse B cell lymphoid neoplasms tested.

Ultroser G can be used at 1% if the cells are not growing well.

Concentration
1mg/ml as standard
Research Area Immunology

References

There are 10 reference entries for this reagent.

View All References

References: 10 entries

Baiardi et al. 2019. Ann Clin Transl Neurol. 6(2):263-273. PMID: 30847359.

Kalsow et al. 2017. Ocul Immunol Inflamm. 25(6):878-883. PMID: 27715362.

Kagami et al. 2017. J Clin Exp Hematop. 57(1):9-14. PMID: 28420813.

van Duijn et al. 2017. J Alzheimers Dis. 60(4):1533-1545. PMID: 29081415.

Breshears et al. 2012. J Biol Chem. 287(39):32578-87. PMID: 22833676.

A disintegrin and metalloproteinase 17 (ADAM17) and epidermal growth factor receptor (EGFR) signaling drive the epithelial response to Staphylococcus aureus toxic shock syndrome toxin-1 (TSST-1).

Europe PMC ID: 22833676

Gatter et al. 1982. Semin Oncol. 9(4):517-25. PMID: 6189189.

The use of monoclonal antibodies for histopathologic diagnosis of human malignancy.

Europe PMC ID: 6189189

Gatter et al. 1982. J Clin Pathol. 35(11):1253-67. PMID: 6183295.

Use of monoclonal antibodies for the histopathological diagnosis of human malignancy.

Europe PMC ID: 6183295


Add a reference

References: 10 entries

Baiardi et al. 2019. Ann Clin Transl Neurol. 6(2):263-273. PMID: 30847359.

Kalsow et al. 2017. Ocul Immunol Inflamm. 25(6):878-883. PMID: 27715362.

Kagami et al. 2017. J Clin Exp Hematop. 57(1):9-14. PMID: 28420813.

van Duijn et al. 2017. J Alzheimers Dis. 60(4):1533-1545. PMID: 29081415.

Breshears et al. 2012. J Biol Chem. 287(39):32578-87. PMID: 22833676.

A disintegrin and metalloproteinase 17 (ADAM17) and epidermal growth factor receptor (EGFR) signaling drive the epithelial response to Staphylococcus aureus toxic shock syndrome toxin-1 (TSST-1).

Gatter et al. 1982. Semin Oncol. 9(4):517-25. PMID: 6189189.

The use of monoclonal antibodies for histopathologic diagnosis of human malignancy.

Gatter et al. 1982. J Clin Pathol. 35(11):1253-67. PMID: 6183295.

Use of monoclonal antibodies for the histopathological diagnosis of human malignancy.


Add a reference